Karacayli Umit, Kaya Ayper, Aydintug Yavuz S, Gunhan Omer
Department of Oral and Maxillofacial Surgery, Gulhane Military Medical Academy, Ankara, Turkey.
Anal Quant Cytol Histol. 2010 Jun;32(3):146-50.
To evaluate the DNA ploidy status of different histologic types of odontogenic tumors (OTs) with epithelial components.
Biopsy materials of cases that were diagnosed as ameloblastoma or OT with epithelial component were retrieved from archives. Routine hematoxylin-eosin sections were prepared from paraffin-embedded tumor materials. A monolayer cell suspension was prepared from tumor cells that were enzymatically and mechanically isolated from biopsy material. DNA content of the nuclei was assessed on Feulgen-stained monolayer cell preparations with automated image cytometry. Ploidy assessments were done according to DNA indices.
Sixty cases of OT were evaluated. All cases had epithelial components. There were 30 pure ameloblastomas and 30 other OTs with epithelial components. All cases were diploid in DNA assessment.
The current study showed that ameloblastomas and other OTs with ameloblastic epithelial components are diploid. To the best of our knowledge, this was the largest study to evaluate the DNA ploidy status of OTs.
评估不同组织学类型的含上皮成分牙源性肿瘤（OTs）的DNA倍体状态。
从档案中检索诊断为成釉细胞瘤或含上皮成分的OT的病例活检材料。用石蜡包埋的肿瘤材料制备常规苏木精-伊红切片。从经酶解和机械分离自活检材料的肿瘤细胞制备单层细胞悬液。用自动图像细胞仪对福尔根染色的单层细胞制剂进行细胞核DNA含量评估。根据DNA指数进行倍体评估。
评估了60例OT。所有病例均有上皮成分。有30例纯成釉细胞瘤和30例其他含上皮成分的OT。所有病例在DNA评估中均为二倍体。
本研究表明，成釉细胞瘤和其他含成釉上皮成分的OT是二倍体。据我们所知，这是评估OTs的DNA倍体状态的最大规模研究。
