First Department of Pathology, Medical School, National and Kapodistrian University of Athens, 75 M. Asias Street, GR 11527, Athens, Greece.
J Oral Pathol Med. 2010 Oct;39(9):687-9. doi: 10.1111/j.1600-0714.2010.00924.x.
Peripheral giant cell granuloma is a tumor of the jaw characterized by the presence of multinucleated giant cells and mononuclear cells within a fibrous stroma. These lesions are considered to be of a reactive nature rather than neoplastic. Although peripheral giant cell granulomas is a well-described clinical entity, little is known on its pathogenesis. The aim of this study was to investigate the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) expression and immunolocalization in giant cell granulomas.
RANKL and OPG protein expression was evaluated in 22 peripheral giant cell granulomas samples, by means of immunohistochemistry. Staining was evaluated semi-quantitatively, according to the extent and intensity of the stain.
RANKL was expressed in all cases with a cytoplasmic staining pattern, whereas OPG expression was detected in 21 of the 22 cases examined. Active multinucleated giant cells exhibited intense immunoreactivity for both proteins.
RANKL and OPG are expressed in peripheral giant cell granulomas of the jaw in a manner supporting the osteoclastic nature of giant cells whereas the possible osteoclastic lineage of stromal monocytes remains ambiguous.
外周性巨细胞肉芽肿是一种颌骨肿瘤，其特征是在纤维基质中有多核巨细胞和单核细胞。这些病变被认为是反应性的，而不是肿瘤性的。尽管外周性巨细胞肉芽肿是一种描述明确的临床实体，但对其发病机制知之甚少。本研究旨在探讨核因子-κB 受体激活剂配体（RANKL）和骨保护素（OPG）在外周性巨细胞肉芽肿中的表达和免疫定位。
采用免疫组织化学法检测 22 例外周性巨细胞肉芽肿样本中 RANKL 和 OPG 蛋白的表达。根据染色的范围和强度进行半定量评估。
RANKL 在所有病例中均呈细胞质染色模式表达，而 OPG 表达在 22 例检查病例中的 21 例中检测到。活性多核巨细胞对这两种蛋白均表现出强烈的免疫反应性。
RANKL 和 OPG 在颌骨的外周性巨细胞肉芽肿中表达，支持巨细胞的破骨细胞特性，而基质单核细胞的可能破骨细胞谱系仍不清楚。
