呼吸道合胞病毒尾部区域的作用：突变对基因组产生和应激颗粒形成的影响。
Roles of the respiratory syncytial virus trailer region: effects of mutations on genome production and stress granule formation.
机构信息
Department of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA.
出版信息
Virology. 2010 Oct 25;406(2):241-52. doi: 10.1016/j.virol.2010.07.006. Epub 2010 Aug 10.
Abstract
The 5' extragenic trailer region of respiratory syncytial virus (RSV) is known to be necessary for genome replication, but is more than three times the length of the 3' leader replication promoter, raising the possibility that trailer might play an additional role in viral replication. To examine this, mutant recombinant viruses were constructed in which the trailer region was truncated or substituted with leader-complement sequence. This analysis showed that the complete trailer increased promoter activity, facilitating genome production and viral multiplication. In addition, trailer-containing viruses did not induce stress granules, whereas the leader-complement virus mutant did, resulting in poor multi-cycle viral growth. These data demonstrate that although the RSV trailer does not contain a unique essential sequence, it augments virus growth by enabling optimal genome production. In addition, a sequence at the 5' terminal end of the trailer region allows RSV to subvert stress granule formation.
摘要
呼吸道合胞病毒（RSV）的 5' 外基因尾区已知是基因组复制所必需的，但长度超过 3' 先导复制启动子三倍以上，这增加了尾区可能在病毒复制中发挥额外作用的可能性。为了研究这一点，构建了突变重组病毒，其中截短或替换了尾区序列。该分析表明，完整的尾区增加了启动子活性，促进了基因组的产生和病毒的增殖。此外，含有尾区的病毒不会诱导应激颗粒，而先导互补病毒突变体则会诱导应激颗粒的形成，导致多轮病毒生长不良。这些数据表明，尽管 RSV 尾区不包含独特的必需序列，但它通过促进最佳基因组的产生来增强病毒的生长。此外，尾区 5' 末端的一个序列允许 RSV 破坏应激颗粒的形成。
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