Saotome K, Morita H, Umeda M
Yokohama City Institute of Health, Takigashira 1-2-17, Isogo-ku, Yokohama 235, Japan.
Toxicol In Vitro. 1989;3(4):317-21. doi: 10.1016/0887-2333(89)90039-8.
A cytotoxicity test with the crystal violet staining method was developed using Chinese hamster lung and HeLa S3 cells in 96-well microtitre plates with an automatic plate reader, to facilitate examination of serial dilutions of the test substance. A good correlation was observed between this procedure and routine cell counting. However, for some chemicals results of the microtitre plate method differed from colony formation counts, particularly when the chemical induced cell stasis. In an application of the microtitre cytotoxicity assay to injection drugs, some drugs that are widely used proved to be very cytotoxic.
采用结晶紫染色法，在96孔微量滴定板中使用中国仓鼠肺细胞和HeLa S3细胞，并借助自动酶标仪开展细胞毒性试验，以利于检测受试物质的系列稀释液。观察到该方法与常规细胞计数之间具有良好的相关性。然而，对于某些化学物质，微量滴定板法的结果与集落形成计数结果不同，尤其是当化学物质诱导细胞停滞时。在将微量滴定细胞毒性试验应用于注射用药物时，一些广泛使用的药物被证明具有很强的细胞毒性。
