从日本患脓皮病的犬中分离出的中间型假葡萄球菌和施氏葡萄球菌凝固酶亚种的抗菌药敏性及耐甲氧西林情况

Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Japan.

作者信息

Kawakami Tetsuji, Shibata Sanae, Murayama Nobuo, Nagata Masahiko, Nishifuji Koji, Iwasaki Toshiroh, Fukata Tsuneo

机构信息

The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.

出版信息

J Vet Med Sci. 2010 Dec;72(12):1615-9. doi: 10.1292/jvms.10-0172. Epub 2010 Aug 10.

DOI:10.1292/jvms.10-0172

PMID:20703027

Abstract

To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 Staphylococcus pseudintermedius (89.5%) and 20 S. schleiferi subsp. coagulans isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in S. pseudintermedius isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In S. schleiferi subsp. coagulans isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC, 70% to MINO, 45% to OFLX or NFLX and 30% to CLDM. None of S. schleiferi subsp. coagulans isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of mecA gene in 66.5% (113 of 170) in S. pseudintermedius and 30.0% (6 of 20) in S. schleiferi subsp. coagulans isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from dogs with pyoderma in Japan.

摘要

为了解日本犬葡萄球菌的物种分布、抗菌药物敏感性趋势和耐甲氧西林情况，从日本两家兽医转诊医院的脓皮病犬中分离出190株凝固酶阳性葡萄球菌（CoPS）。采用多重聚合酶链反应（M-PCR）方法，鉴定出两种CoPS物种：170株中间型葡萄球菌（89.5%）和20株施氏葡萄球菌凝固亚种分离株（10.5%）。对这些分离株测定了对7种抗菌药物的敏感性。总体而言，中间型葡萄球菌分离株对头孢氨苄（CEX）、阿莫西林/克拉维酸（CVA/AMPC）、米诺环素（MINO）、氧氟沙星（OFLX）、诺氟沙星（NFLX）、林可霉素（LCM）和克林霉素（CLDM）的敏感率分别为38.2%、52.4%、34.7%、31.2%、34.1%、1.2%和11.2%。在施氏葡萄球菌凝固亚种分离株中，55%对CEX敏感，80%对CVA/AMPC敏感，70%对MINO敏感，45%对OFLX或NFLX敏感，30%对CLDM敏感。施氏葡萄球菌凝固亚种分离株对LCM均不敏感。为确定耐甲氧西林菌株的流行情况，我们采用了一种PCR方法，该方法能够检测到中间型葡萄球菌中66.5%（170株中的113株）和施氏葡萄球菌凝固亚种分离株中30.0%（20株中的6株）的mecA基因片段。耐甲氧西林的CoPS对CEX、CVA/AMPC、OFLX、NFLX和CLDM的敏感率显著低于甲氧西林敏感的CoPS分离株。这些数据表明，从日本脓皮病犬中分离出的葡萄球菌耐甲氧西林水平较高。