Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, National University of San Marcos, Lima, Perú.
Open Vet J. 2021 Jul-Sep;11(3):364-369. doi: 10.5455/OVJ.2021.v11.i3.5. Epub 2021 Jul 20.
Dermatological infections are the most common cases in the daily pet clinic. Since its discovery in 1990, subspecies have been reported more frequently in canine otitis externa and pyoderma and even in cases of zoonoses.
Detect the presence of subsp. of canine otitis externa and pyoderma, its antimicrobial resistance, and the presence of Agen.
Three-hundred-thirty-one swabs from dogs with otitis externa and pyoderma were cultured on bacteriological agar for bacterial isolation and subsequent biochemical and molecular identification. The identified subsp. were evaluated for their antimicrobial susceptibility using the Kirby-Bauer technique, including an oxacillin disk, and subsequently, a PCR was run to identify which ones had the A gene.
Thirty-four (22.97%) and twelve (6.56%) isolates were identified as subspecies from otitis externa and pyoderma, respectively. Fluoroquinolones, the most widely used group of antibiotics in Peru, showed a susceptibility of 58.82% (20/34) in cases of otitis externa and 50% (6/12) in cases of canine pyoderma. Meanwhile, nitrofurantoin was the antibiotic with the best efficacy in both cases, with 97% (33/34) in otitis externa and 83% (10/12) in pyoderma. Furthermore, 40% (13/34) of subsp. isolated from otitis externa were resistant to methicillin, and 85.29% (29/34) had the A gene. On the other hand, the only methicillin-resistant isolate from pyoderma was also the only one with a A gene.
This study is the first report of subsp. in Peru, finding a higher percentage than reported in other South American countries.
皮肤科感染是日常宠物诊所中最常见的病例。自 1990 年发现以来，亚种在犬外耳炎和脓皮病中更为频繁地被报道，甚至在人畜共患病病例中也有报道。
检测犬外耳炎和脓皮病中亚种的存在、其对抗菌药物的耐药性以及 Agen 的存在。
对 331 份来自患有外耳炎和脓皮病的犬的拭子进行细菌培养，在细菌琼脂上进行细菌分离，随后进行生化和分子鉴定。对鉴定出的亚种进行药敏试验，采用 Kirby-Bauer 技术，包括一个苯唑西林圆盘，随后进行 PCR 以鉴定哪些具有 A 基因。
从外耳炎和脓皮病中分别鉴定出 34（22.97%）和 12（6.56%）株亚种。氟喹诺酮类药物是秘鲁最广泛使用的抗生素组，在外耳炎病例中的敏感性为 58.82%（20/34），在犬脓皮病病例中的敏感性为 50%（6/12）。同时，在两种情况下，呋喃妥因是疗效最好的抗生素，在外耳炎中的疗效为 97%（33/34），在脓皮病中的疗效为 83%（10/12）。此外，40%（13/34）从外耳炎分离的亚种对甲氧西林耐药，85.29%（29/34）具有 A 基因。另一方面，从脓皮病中分离出的唯一耐甲氧西林的菌株也唯一具有 A 基因。
本研究是在秘鲁首次报道亚种，发现的比例高于在其他南美国家报道的比例。
