Leesombun Arpron, Sungpradit Sivapong, Bangphoomi Norasuthi, Thongjuy Orathai, Wechusdorn Jantraporn, Riengvirodkij Sunee, Wannawong Jakaphan, Boonmasawai Sookruetai
Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Pharmaceuticals (Basel). 2023 May 12;16(5):741. doi: 10.3390/ph16050741.
Emergence of methicillin-resistant (MRSP) isolated from dogs with cutaneous and wound infections has significantly impacted veterinary medicine. This study aimed to isolate from canine pyoderma and investigate the effects of ethanolic extracts of (PB), (PS), and (PN) on the bacterial growth and biofilm formation of and MRSP. Of the isolated 152 isolates, 53 were identified as using polymerase chain reaction, and 10 isolates (6.58%) were identified as MRSP based on the presence of . Based on phenotype, 90% of MRSPs were multidrug-resistant. All MRSP had moderate (10%, 1/10) and strong (90%, 9/10) biofilm production ability. PB extracts were the most effective in inhibiting planktonic cells, and the minimum inhibitory concentration at which ≥50% of the isolates were inhibited (MIC) was 256 µg/mL (256-1024 µg/mL) for isolates and 512 µg/mL (256-1024 µg/mL) for MRSP isolates. The MIC for and MRSP was 512 µg/mL. In XTT assay, PB at 4× MIC showed an inhibition rate of 39.66-68.90% and 45.58-59.13% for and MRSP, respectively, in inhibiting biofilm formation. For PB at 8× MIC, the inhibition rates for and MRSP were 50.74-81.66% and 59.57-78.33%, respectively. Further, 18 compounds were identified in PB using gas chromatography-mass spectrometry, and hydroxychavicol (36.02%) was the major constituent. These results indicated that PB could inhibit bacteria growth of and biofilm formation by and MRSP isolated from canine pyoderma in a concentration-dependent manner. Therefore, PB is a potential candidate for the treatment of MRSP infection and biofilm formation in veterinary medicine.
从患有皮肤和伤口感染的犬类中分离出的耐甲氧西林金黄色葡萄球菌（MRSP）的出现对兽医学产生了重大影响。本研究旨在从犬脓皮病中分离出金黄色葡萄球菌，并研究蜂胶（PB）、紫锥菊提取物（PS）和紫锥菊（PN）的乙醇提取物对金黄色葡萄球菌和MRSP细菌生长及生物膜形成的影响。在分离出的152株菌株中，53株通过聚合酶链反应鉴定为金黄色葡萄球菌，基于mecA基因的存在，10株（6.58%）被鉴定为MRSP。基于表型，90%的MRSP具有多重耐药性。所有MRSP均具有中度（10%，1/10）和强（90%，9/10）的生物膜产生能力。PB提取物对浮游细胞的抑制作用最为有效，对于金黄色葡萄球菌分离株，≥50%的分离株被抑制时的最低抑菌浓度（MIC）为256µg/mL（256 - 1024µg/mL），对于MRSP分离株为512µg/mL（256 - 1024µg/mL）。金黄色葡萄球菌和MRSP的MIC均为512µg/mL。在XTT试验中，4倍MIC的PB对金黄色葡萄球菌和MRSP生物膜形成的抑制率分别为39.66 - 68.90%和45.58 - 59.13%。对于8倍MIC的PB，对金黄色葡萄球菌和MRSP的抑制率分别为50.74 - 81.66%和59.57 - 78.33%。此外，使用气相色谱 - 质谱法在PB中鉴定出18种化合物，其中羟基查耳酮（36.02%）为主要成分。这些结果表明，PB可以浓度依赖性方式抑制从犬脓皮病中分离出的金黄色葡萄球菌和MRSP的细菌生长及生物膜形成。因此，PB是兽医学中治疗MRSP感染和生物膜形成的潜在候选药物。
