Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, Virginia 22904, USA.
ACS Nano. 2010 Sep 28;4(9):4989-96. doi: 10.1021/nn901873t.
Responsive biomaterials play important roles in imaging, diagnostics, and therapeutics. Polymeric nanoparticles (NPs) containing hydrophobic and hydrophilic segments are one class of biomaterial utilized for these purposes. The incorporation of luminescent molecules into NPs adds optical imaging and sensing capability to these vectors. Here we report on the synthesis of dual-emissive, pegylated NPs with "stealth"-like properties, delivered intravenously (IV), for the study of tumor accumulation. The NPs were created by means of stereocomplexation using a methoxy-terminated polyethylene glycol and poly(D-lactide) (mPEG-PDLA) block copolymer combined with iodide-substituted difluoroboron dibenzoylmethane-poly(L-lactide) (BF2dbm(I)PLLA). Boron nanoparticles (BNPs) were fabricated in two different solvent compositions to study the effects on BNP size distribution. The physical and photoluminescent properties of the BNPs were studied in vitro over time to determine stability. Finally, preliminary in vivo results show that stereocomplexed BNPs injected IV are taken up by tumors, an important prerequisite to their use as hypoxia imaging agents in preclinical studies.
响应性生物材料在成像、诊断和治疗中发挥着重要作用。含有疏水和亲水部分的聚合物纳米颗粒（NPs）是用于这些目的的一类生物材料。将发光分子掺入 NPs 中为这些载体增加了光学成像和传感能力。在这里，我们报告了一种具有“隐形”特性的双发射、聚乙二醇化 NPs 的合成，通过静脉内（IV）给药，用于研究肿瘤积累。这些 NPs 是通过使用甲氧基封端的聚乙二醇和聚（D-丙交酯）（mPEG-PDLA）嵌段共聚物与碘取代的二氟硼二苯甲酰甲烷-聚（L-丙交酯）（BF2dbm（I）PLLA）进行立体复合形成的。在两种不同的溶剂组成中制备了硼纳米颗粒（BNPs），以研究对 BNPs 尺寸分布的影响。体外随着时间的推移研究了 BNPs 的物理和光致发光性质，以确定稳定性。最后，初步的体内结果表明，静脉内注射的立体复合 BNPs 被肿瘤吸收，这是它们在临床前研究中用作缺氧成像剂的重要前提。
