Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
J Org Chem. 2010 Aug 20;75(16):5733-6. doi: 10.1021/jo100757t.
Phosphitylation of the side chain hydroxyl function of Fmoc protected tyrosine with 5'-phosphoramidites of suitably protected cytidine, adenosine, and guanosine, followed by oxidation gave three novel nucleotidylated amino acid building blocks. After protective group manipulation, these building blocks were used in a solid phase peptide synthesis to afford the nucleotidylated poliovirus proteins VPgpC, VPgpA, and VPgpG.
用适当保护的胞苷、腺苷和鸟苷的 5'-磷酰胺保护的核苷将 Fmoc 保护的酪氨酸的侧链羟基磷酸化，然后氧化得到三个新型核苷酸化氨基酸砌块。经过保护基操作后，这些砌块被用于固相肽合成，得到核苷酸化的脊髓灰质炎病毒蛋白 VPgpC、VPgpA 和 VPgpG。
