David H. Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Mol Cell. 2010 Aug 13;39(3):444-54. doi: 10.1016/j.molcel.2010.07.032.
How spatial information is translated into a chemical signal is a fundamental problem in all organisms. The spindle position checkpoint is a prime example of this problem. This checkpoint senses spindle position and, in budding yeast, inhibits the mitotic exit network (MEN), a signaling pathway that promotes exit from mitosis. We find that spindle position is sensed by a system composed of MEN-inhibitory and -activating zones and a sensor that moves between them. The MEN inhibitory zone is located in the mother cell, the MEN-activating zone in the bud, and the spindle pole body (SPB), where the components of the MEN reside, functions as the sensor. Only when an SPB escapes the MEN inhibitor Kin4 in the mother cell and moves into the bud where the MEN activator Lte1 resides can exit from mitosis occur. In this manner, spatial information is sensed and translated into a chemical signal.
空间信息如何转化为化学信号是所有生物的一个基本问题。纺锤体位置检查点就是这个问题的一个典型例子。这个检查点感知纺锤体的位置，在出芽酵母中，它抑制有丝分裂退出网络（MEN），这是一个促进有丝分裂退出的信号通路。我们发现，纺锤体的位置是由一个由 MEN 抑制区和激活区以及在它们之间移动的传感器组成的系统来感知的。 MEN 抑制区位于母细胞中， MEN 激活区位于芽中，纺锤体极体（SPB）， MEN 的成分所在的地方，作为传感器发挥作用。只有当 SPB 从母细胞中逃避 MEN 抑制剂 Kin4 并移动到含有 MEN 激活剂 Lte1 的芽中时，有丝分裂才能退出。通过这种方式，空间信息被感知并转化为化学信号。
