[采用反相高效液相色谱法测定不同产地野菊花中绿原酸、咖啡酸和木犀草苷的含量]

[Determination of chlorogenic acid, caffeic acid and linarin in Flos Chrysanthemi Indici from different places by RP-hPLC].

作者信息

Guo Qiaosheng, Fang Hailing, Shen Haijin

机构信息

Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 May;35(9):1160-3. doi: 10.4268/cjcmm20100917.

DOI:10.4268/cjcmm20100917

PMID:20707074

Abstract

OBJECTIVE

To evaluate the quality of Flos Chrysanthemi Indici which produced in twenty-two different producing places.

METHOD

Chlorogenic acid and caffeic acid were analyzed on a Shim-pack C8 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-0.5% phosphoric acid( 19:81). The detection wavelength was set at 326 nm. Linarin were eluted with the mobile phase consisted of methanol-water-acetic acid(26: 23: 1). The detection wavelength was set at 334 nm. The column temperature was 25 degrees C. The flow rate was 1.0 mL x min .

RESULT

The linear response ranged within 2.5-50 microg for chlorogenic acid (r = 0.998), 2.5-25 microg for caffeic acid (r = 0.998) and 4.97-41.47 microg for linarin (r = 0.999), respectively. Recoveries were 100.8% with RSD 2.1% for chlorogenic acid, 96.2% with RSD 2.3% for caffeic acid and 103.7% with RSD 1.8% for linarin.

CONCLUSION

There was a significant difference in the content of chlorogenic acid, caffeic acid, linarin among the samples. The content of chlorogenic in the sample from Fengdou Chongqing city was the highest in those from other places. The content of caffeic acid in the all samples is very low. The content of linarin in the samples from Jiangsu province and Anhui province almost reached the national standard in pharmacopoeia.

摘要

目的

评价二十二个不同产地所产野菊花的质量。

方法

采用Shim-pack C8柱（4.6 mm×250 mm，5μm）分析绿原酸和咖啡酸，流动相为乙腈-0.5%磷酸（19:81）。检测波长设定为326 nm。采用流动相甲醇-水-乙酸（26:23:1）洗脱蒙花苷。检测波长设定为334 nm。柱温为25℃。流速为1.0 mL·min⁻¹。

结果

绿原酸线性响应范围在2.5 - 50μg之间（r = 0.998），咖啡酸在2.5 - 25μg之间（r = 0.998），蒙花苷在4.97 - 41.47μg之间（r = 0.999）。绿原酸回收率为100.8%，RSD为2.1%；咖啡酸回收率为96.2%，RSD为2.3%；蒙花苷回收率为103.7%，RSD为1.8%。