[高效液相色谱法同时测定杭白菊中6种活性成分]
[Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC].
作者信息
Qin Shan, Wen Xuesen
机构信息
Institute of Pharmacognosy, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(11):1474-7.
OBJECTIVE
To develop a HPLC method quantitative method for simultaneous determination of chlorogenic acid, 1, 5-dicaffeoylquinic acid, isochlorogenic acid A, isochlorogenic acid C, luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Chrysanthemum morifolium Ramat.
METHOD
A Phenomenex Gemini-NX C18 column (4.6 mm x 250 mm, 5 microm) was used with CH3 OH and 0.4% H3PO4 as mobile phases. The flow rate was 1 mL x min(-1), the column temperature was 25 degrees C, and the detection wavelength was set at 350 nm.
RESULT
The 6 active components were in baseline separation. The linearity of this method was good (r > or = 0.999 7), and the average recoveries were 100.6% - 102.4%, RSD < 3%. Except isochlorogenic acid A, the contents of the determined components in the steam-blanched flower heads were significantly higher than those non blanched. The contents of chlorogenic acid and isochlorogenic acid A in the steam-blanched semiopened flower heads were higher than fully opened ones by 53% and 41%, respectively.
CONCLUSION
The method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Chrysanthemum.
目的
建立高效液相色谱法同时测定菊花中绿原酸、1,5-二咖啡酰奎宁酸、异绿原酸A、异绿原酸C、木犀草素-7-O-β-D-葡萄糖苷和芹菜素-7-O-β-D-葡萄糖苷的定量方法。
方法
采用菲罗门Gemini-NX C18柱（4.6 mm×250 mm，5μm），以甲醇和0.4%磷酸为流动相。流速为1 mL·min⁻¹，柱温为25℃，检测波长设定为350 nm。
结果
6种活性成分达到基线分离。该方法线性关系良好（r≥0.999 7），平均回收率为100.6% - 102.4%，相对标准偏差（RSD）<3%。除异绿原酸A外，蒸制过的菊花头状花序中各测定成分含量显著高于未蒸制的。蒸制过的半开放菊花头状花序中绿原酸和异绿原酸A的含量分别比完全开放的高53%和41%。
结论
该方法灵敏、准确、可靠、重复性好，可用于菊花的质量评价。
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