Liu Fei, Yang Yang, Tan Xiaojie, Bi Kaishun, Jia Ying
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(16):2067-70.
To develop an HPLC method for the determination of luteolin-7-O-glycoside, apigenin-7-O-glycoside, linarin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.
The reversed phase HPLC system consisting of a C18 column (4.6 mm x 250 mm, 5 microm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted. The absorbance was monitored at 326 nm.
The linear response range was 1.08-21.5 mg x L(-1) (r = 0.9990), 0.278-5.57 mg x L(-1) (r = 0.9994), 5.58-112 mg x L(-1) (r = 0.9999), 0.521-10.4 mg x L(-1) (r = 0.9995), 0.162-3.25 mg x L(-1) (r = 0.9999) and 0.288-5.76 mg x L(-1) (r = 0.9998), respectively (n = 6). The average recoveries (n = 9) of six flavonoids were 96.34%-104.0%. All of RSD of precision (n = 6) and repeatability (n = 6) were less than 1.2% and 2.6%.
The validation data demonstrated that the method was accurate and repeatable, and can be ase to measure the six flavonoids in Flos Chrysanthemi Indici.
建立同时测定野菊花中木犀草素-7-O-糖苷、芹菜素-7-O-糖苷、蒙花苷、木犀草素、芹菜素和刺槐素的高效液相色谱法。
采用反相高效液相色谱系统，以C18柱（4.6 mm×250 mm，5μm）为固定相，乙腈和0.05%磷酸混合溶液为流动相进行梯度洗脱，检测波长为326 nm。
6种黄酮类成分的线性范围分别为1.08 - 21.5 mg·L⁻¹（r = 0.9990）、0.278 - 5.57 mg·L⁻¹（r = 0.9994）、5.58 - 112 mg·L⁻¹（r = 0.9999）、0.521 - 10.4 mg·L⁻¹（r = 0.9995）、0.162 - 3.25 mg·L⁻¹（r = 0.9999）和0.288 - 5.76 mg·L⁻¹（r = 0.9998）（n = 6）。6种黄酮类成分的平均回收率（n = 9）为96.34% - 104.0%。精密度（n = 6）和重复性（n = 6）的RSD均小于1.2%和2.6%。
该方法经方法学验证，结果准确、重复性好，可用于测定野菊花中6种黄酮类成分的含量。
