[反相高效液相色谱法同时测定野菊花中六种黄酮类化合物]

[Simultaneous determination of six flavonoids in Flos Chrysanthemi Indici by RP-HPLC].

作者信息

Liu Fei, Yang Yang, Tan Xiaojie, Bi Kaishun, Jia Ying

机构信息

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(16):2067-70.

PMID:19938548

Abstract

OBJECTIVE

To develop an HPLC method for the determination of luteolin-7-O-glycoside, apigenin-7-O-glycoside, linarin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.

METHOD

The reversed phase HPLC system consisting of a C18 column (4.6 mm x 250 mm, 5 microm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted. The absorbance was monitored at 326 nm.

RESULT

The linear response range was 1.08-21.5 mg x L(-1) (r = 0.9990), 0.278-5.57 mg x L(-1) (r = 0.9994), 5.58-112 mg x L(-1) (r = 0.9999), 0.521-10.4 mg x L(-1) (r = 0.9995), 0.162-3.25 mg x L(-1) (r = 0.9999) and 0.288-5.76 mg x L(-1) (r = 0.9998), respectively (n = 6). The average recoveries (n = 9) of six flavonoids were 96.34%-104.0%. All of RSD of precision (n = 6) and repeatability (n = 6) were less than 1.2% and 2.6%.

CONCLUSION

The validation data demonstrated that the method was accurate and repeatable, and can be ase to measure the six flavonoids in Flos Chrysanthemi Indici.

摘要

目的

建立同时测定野菊花中木犀草素-7-O-糖苷、芹菜素-7-O-糖苷、蒙花苷、木犀草素、芹菜素和刺槐素的高效液相色谱法。

方法

采用反相高效液相色谱系统，以C18柱（4.6 mm×250 mm，5μm）为固定相，乙腈和0.05%磷酸混合溶液为流动相进行梯度洗脱，检测波长为326 nm。

结果

6种黄酮类成分的线性范围分别为1.08 - 21.5 mg·L⁻¹（r = 0.9990）、0.278 - 5.57 mg·L⁻¹（r = 0.9994）、5.58 - 112 mg·L⁻¹（r = 0.9999）、0.521 - 10.4 mg·L⁻¹（r = 0.9995）、0.162 - 3.25 mg·L⁻¹（r = 0.9999）和0.288 - 5.76 mg·L⁻¹（r = 0.9998）（n = 6）。6种黄酮类成分的平均回收率（n = 9）为96.34% - 104.0%。精密度（n = 6）和重复性（n = 6）的RSD均小于1.2%和2.6%。