[反相高效液相色谱法测定野菊花中槲皮素、木犀草素、芹菜素和刺槐素的含量]

[Determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici by RP-HPLC].

作者信息

Shen Haijin, Guo Qiaosheng, Fang Hailing, Wang Yanru, Jin Miao

机构信息

Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Jan;35(2):191-3. doi: 10.4268/cjcmm20100216.

DOI:10.4268/cjcmm20100216

PMID:20394292

Abstract

OBJECTIVE

To develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici.

METHOD

An Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 350 nm.

RESULT

The linear response ranged from 1.02-20.48 mg x L(-1) for quercetin (r = 0.9994, n = 5), 1.03-20.54 mg x L(-1) for luteolin (r = 0.9992, n = 5), 1.12-22.40 mg x L(-1) for apigenin (r = 0.9995, n = 5), 1.01-20.22 mg x L(-1) for acacetin (r = 0.9998, n = 5), respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference (alpha = 0.01) among the contents of four flavonoids and total flavonoids was found.

CONCLUSION

The method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.

摘要

目的

建立一种反相高效液相色谱法（RP-HPLC）测定野菊花中槲皮素、木犀草素、芹菜素和刺槐素的含量。

方法

采用Eclipse XDB-C18柱（4.6 mm×250 mm，5 µm），于25℃，以甲醇-0.2%磷酸为流动相进行梯度洗脱。流速设定为1.0 mL·min⁻¹。检测波长为350 nm。

结果

槲皮素线性响应范围为1.02 - 20.48 mg·L⁻¹（r = 0.9994，n = 5），木犀草素为1.03 - 20.54 mg·L⁻¹（r = 0.9992，n = 5），芹菜素为1.12 - 22.40 mg·L⁻¹（r = 0.9995，n = 5），刺槐素为1.01 - 20.22 mg·L⁻¹（r = 0.9998，n = 5）。槲皮素回收率为101.3%，相对标准偏差（RSD）为1.3%；木犀草素回收率为100.62%，RSD为1.4%；芹菜素回收率为98.42%，RSD为1.7%；刺槐素回收率为99.02%，RSD为0.8%。四种黄酮类化合物及总黄酮含量之间存在显著差异（α = 0.01）。