Institute of Basic Medical Sciences, Department of Physiology, University of Oslo, Norway.
J Neurosci Methods. 2010 Oct 15;192(2):254-60. doi: 10.1016/j.jneumeth.2010.08.003. Epub 2010 Aug 11.
We describe a new cable-free, non-telemetric method for synchronized electrophysiological and video recordings of seizure activity in freely moving mice. The electrophysiological recordings were made by a head-mounted 4-channel data-logging device, allowing the mouse to move freely in its cage, and even to be moved from cage to cage under ongoing recording. Seizures were studied in Synapsin I/II double knock-out (SynDKO) mice, a genetically engineered mouse line that shows seizures upon daily handling procedures such as tail lifting during cage changes, much in resemblance to the more studied El mouse. The ability to elicit seizures through daily handling in SynDKO mice undergoing electrophysiological recording is a significant improvement in comparison to the traditional cable-based set-up. Furthermore, with its four channels and a sample rate of up to 500Hz, the data-logging device opens for more varied electrophysiological studies than other available cable-free systems.
我们描述了一种新的无缆、非遥测方法，用于在自由活动的小鼠中同步记录癫痫发作的电生理和视频。电生理记录是通过一个头戴的 4 通道数据记录设备进行的，允许小鼠在笼子里自由移动，甚至可以在持续记录的情况下从一个笼子移动到另一个笼子。在突触素 I/II 双重敲除（SynDKO）小鼠中研究了癫痫发作，这是一种基因工程小鼠系，在日常处理过程中如在更换笼子时提起尾巴会出现癫痫发作，与更具研究价值的 El 小鼠非常相似。与传统的基于电缆的设置相比，在接受电生理记录的 SynDKO 小鼠中通过日常处理引发癫痫发作的能力是一个重大改进。此外，该数据记录设备具有四个通道和高达 500Hz 的采样率，比其他可用的无缆系统更适合进行各种电生理研究。
