Vogler Emily C, Flynn Daniel T, Busciglio Federico, Bohannan Ryan C, Tran Alison, Mahavongtrakul Matthew, Busciglio Jorge A
Institute for Memory Impairment and Neurological Disorders, University of California, Irvine, Irvine, CA, United States.
School of Education, University of California, Irvine, Irvine, CA, United States.
Front Neurosci. 2017 Nov 14;11:629. doi: 10.3389/fnins.2017.00629. eCollection 2017.
Wireless electroencephalography (EEG) of small animal subjects typically utilizes miniaturized EEG devices which require a robust recording and electrode assembly that remains in place while also being well-tolerated by the animal so as not to impair the ability of the animal to perform normal living activities or experimental tasks. We developed simple and fast electrode assembly and method of electrode implantation using electrode wires and wire-wrap technology that provides both higher survival and success rates in obtaining recordings from the electrodes than methods using screws as electrodes. The new wire method results in a 51% improvement in the number of electrodes that successfully record EEG signal. Also, the electrode assembly remains affixed and provides EEG signal for at least a month after implantation. Screws often serve as recording electrodes, which require either drilling holes into the skull to insert screws or affixing screws to the surface of the skull with adhesive. Drilling holes large enough to insert screws can be invasive and damaging to brain tissue, using adhesives may interfere with conductance and result in a poor signal, and soldering screws to wire leads results in fragile connections. The methods presented in this article provide a robust implant that is minimally invasive and has a significantly higher success rate of electrode implantation. In addition, the implant remains affixed and produces good recordings for over a month, while using economical, easily obtained materials and skills readily available in most animal research laboratories.
小动物受试者的无线脑电图（EEG）通常使用小型化的EEG设备，这需要一个坚固的记录和电极组件，该组件要保持在原位，同时还要让动物能够很好地耐受，以免损害动物进行正常生活活动或实验任务的能力。我们开发了一种简单快速的电极组件和电极植入方法，使用电极线和绕线技术，与使用螺丝作为电极的方法相比，该方法在从电极获取记录方面具有更高的存活率和成功率。新的线法使成功记录EEG信号的电极数量提高了51%。此外，电极组件在植入后至少一个月内保持固定并提供EEG信号。螺丝通常用作记录电极，这需要在头骨上钻孔以插入螺丝，或者用粘合剂将螺丝固定到头骨表面。钻足够大的孔来插入螺丝可能具有侵入性并会损害脑组织，使用粘合剂可能会干扰导电性并导致信号不佳，而将螺丝焊接到电线引线上会导致连接脆弱。本文介绍的方法提供了一种侵入性最小且电极植入成功率显著更高的坚固植入物。此外，该植入物在一个多月的时间里保持固定并能产生良好的记录，同时使用的是大多数动物研究实验室都容易获得的经济实惠的材料和技术。
