Department of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
Biochem Biophys Res Commun. 2010 Oct 1;400(4):461-5. doi: 10.1016/j.bbrc.2010.08.029. Epub 2010 Aug 12.
Adipose tissue-derived mesenchymal stem cells (ASCs) have been reported to be multipotent and to differentiate into various cell types, including osteocytes, adipocytes, chondrocytes, and neural cells. Recently, many authors have reported that ASCs are also able to differentiate into vascular endothelial cells (VECs) in vitro. However, these reports included the use of medium containing fetal bovine serum for endothelial differentiation. In the present study, we have developed a novel method for differentiating mouse ASCs into VECs under serum-free conditions. After the differentiation culture, over 80% of the cells expressed vascular endothelial-specific marker proteins and could take up low-density lipoprotein in vitro. This protocol should be helpful in clarifying the mechanisms of ASC differentiation into the VSC lineage.
脂肪组织来源的间充质干细胞（ASCs）已被报道具有多能性，并能分化为多种细胞类型，包括成骨细胞、脂肪细胞、软骨细胞和神经细胞。最近，许多作者报道说，ASCs 也能够在体外分化为血管内皮细胞（VECs）。然而，这些报告包括使用含有胎牛血清的培养基进行内皮细胞分化。在本研究中，我们开发了一种在无血清条件下将小鼠 ASCs 分化为 VECs 的新方法。分化培养后，超过 80%的细胞表达血管内皮特异性标记蛋白，并能在体外摄取低密度脂蛋白。该方案有助于阐明 ASC 分化为 VSC 谱系的机制。
