Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba.
Int J Med Microbiol. 2011 Jan;301(1):16-25. doi: 10.1016/j.ijmm.2010.04.020. Epub 2010 Aug 14.
Neisseria meningitidis causes meningitis and septicemia. There is no single vaccine against all serogroup B meningococcal (MenB) strains up to now. Their capsular polysaccharide (MenB CPS) bears epitopes both cross-reacting and non-cross-reactive with human polysialic acid. A bactericidal and protective antibody mAb (13D9) recognizing a unique epitope in MenB CPS was used to screen a phage-displayed peptide library. Four peptides, able to bind mAb 13D9 in competition with MenB CPS, were identified. Immunization of mice with the phage-displayed peptides elicited anti-peptide IgG antibodies, mainly IgG(2a) for 3 of the peptides and bactericidal and protective antibody levels for one of them. Peptides specifically targeting the immune response toward epitopes found only in MenB CPS could be considered for a universal vaccine against serogroup B meningococcal strains.
脑膜炎奈瑟菌可引起脑膜炎和败血症。目前尚无针对所有 B 群脑膜炎奈瑟菌（MenB）菌株的单一疫苗。它们的荚膜多糖（MenB CPS）具有与人类多涎酸既有交叉反应又无交叉反应的表位。一种杀菌和保护性抗体单克隆抗体（13D9）识别 MenB CPS 中的独特表位，用于筛选噬菌体展示肽文库。鉴定出四个能够与 MenB CPS 竞争结合 mAb 13D9 的肽。用噬菌体展示的肽免疫小鼠可引起抗肽 IgG 抗体，其中 3 种肽主要产生 IgG(2a)，而其中 1 种肽可产生杀菌和保护性抗体水平。针对仅在 MenB CPS 中发现的表位的靶向免疫反应的肽可被视为针对 B 群脑膜炎奈瑟菌菌株的通用疫苗。
