Lehman N, Wayne R K
Department of Biology, University of California, Los Angeles 90024-1606.
Genetics. 1991 Jun;128(2):405-16. doi: 10.1093/genetics/128.2.405.
A restriction-site survey of 327 coyotes (Canis latrans) from most parts of their North American range reveals 32 mitochondrial DNA (mtDNA) genotypes. The genotypes are not strongly partitioned in space, suggesting that there is high gene flow among coyote subpopulations. Consequently, each new geographic location added to the study has a decreasing probability of containing a mtDNA genotype that had not been previously discovered. This being the case, by using Monte Carlo sampling experiments, we can estimate the total number of genotypes that would be found if all possible localities were surveyed. This estimate of total genotypic variability agrees qualitatively with estimates based on theoretical considerations of the expected number of alleles in a stable population. We also predict effective population sizes from genotype data. The accuracy of these estimates is thought to be dependent on the fact that coyotes are not highly genetically structured, a situation which may apply to highly mobile species.
对来自北美大部分分布区域的327只郊狼（犬属）进行的限制性酶切位点调查揭示了32种线粒体DNA（mtDNA）基因型。这些基因型在空间上没有明显的划分，这表明郊狼亚种群之间存在高度的基因流动。因此，每一个新加入研究的地理位置包含先前未发现的mtDNA基因型的概率都在降低。在这种情况下，通过蒙特卡洛抽样实验，我们可以估计如果对所有可能的地点进行调查会发现的基因型总数。这种对总基因型变异性的估计在定性上与基于稳定种群中等位基因预期数量的理论考虑所做的估计一致。我们还根据基因型数据预测有效种群大小。这些估计的准确性被认为取决于郊狼没有高度遗传结构这一事实，这种情况可能适用于高度移动的物种。
