Nanu A, Taneja A
Blood Bank, All India Institute of Medical Sciences, New Delhi.
Indian J Med Res. 1991 Feb;94:80-2.
Fibronectin was extracted from 20 units of 3 days old plasma by the heparin cold precipitation technique using 15 or 30 units of sodium heparin per ml of plasma. Immunoelectrophoresis of the extracted fibronectin showed it to be 131.37 +/- 24.73 times concentrated over fibronectin levels in standard plasma, with a mean recovery of 73.99 +/- 18.40 per cent. Corresponding figures by ELISA were 118.95 +/- 27.99 (concentration) and 73.99 +/- 18.40 (per cent).
采用肝素冷沉淀技术，每毫升血浆使用15或30单位肝素钠，从20单位3日龄血浆中提取纤连蛋白。对提取的纤连蛋白进行免疫电泳显示，其浓度是标准血浆中纤连蛋白水平的131.37±24.73倍，平均回收率为73.99±18.40%。酶联免疫吸附测定法（ELISA）的相应数据为118.95±27.99（浓度）和73.99±18.40（百分比）。
