Tien S L, Ong Y W, Lim S H, Chiang T
National Blood Centre, Singapore Blood Transfusion Service.
Ann Acad Med Singap. 1990 Nov;19(6):831-2.
Fibronectin prepared by heparin-cold-precipitation from 10 packs each of 24-hour plasma, stored plasma and 6-hour frozen plasma was assayed by electroimmunodiffusion. Its yield obtained from 24-hour plasma was significantly higher than that from stored plasma (p less than 0.01) indicating a decline with storage. Freezing of plasma within 6 hours retards degradation of fibronectin. Therefore, 24-hour plasma or 6-hour frozen plasma should be used to prepare fibronectin in order to achieve a better yield.
采用肝素冷沉淀法从10份24小时血浆、库存血浆和6小时冷冻血浆（每份10袋）中制备纤连蛋白，并用免疫电泳法进行测定。从24小时血浆中获得的纤连蛋白产量显著高于库存血浆（p<0.01），表明其产量随储存时间下降。6小时内冷冻血浆可延缓纤连蛋白的降解。因此，为获得更高产量，应使用24小时血浆或6小时冷冻血浆来制备纤连蛋白。
