肝窦内皮细胞和胆管细胞在照射和部分肝切除术后的再增殖。
Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
机构信息
Department of General Surgery, University Medical Centre Goettingen, Robert-Koch-Strasse 40, Goettingen, Germany.
出版信息
World J Gastroenterol. 2010 Aug 21;16(31):3928-35. doi: 10.3748/wjg.v16.i31.3928.
AIM
To investigate whether irradiation (IR) and partial hepatectomy (PH) may prepare the host liver for non-parenchymal cell (NPC) transplantation.
METHODS
Livers of dipeptidyl peptidase IV (DPPIV)-deficient rats were pre-conditioned with external beam IR (25 Gy) delivered to two-thirds of the right liver lobules followed by a one-third PH of the untreated lobule. DPPIV-positive liver cells (NPC preparations enriched for liver sinusoidal endothelial cells (LSECs) and hepatocytes) were transplanted via the spleen into the recipient livers. The extent and quality of donor cell engraftment and growth was studied over a long-term interval of 16 wk after transplantation.
RESULTS
Host liver staining demonstrated 3 different repopulation types. Well defined clusters of donor-derived hepatocytes with canalicular expression of DPPIV were detectable either adjacent to or in between large areas of donor cells (covering up to 90% of the section plane) co-expressing the endothelial marker platelet endothelial cell adhesion molecule. The third type consisted of formations of DPPIV-positive duct-like structures which co-localized with biliary epithelial CD49f.
CONCLUSION
Liver IR and PH as a preconditioning stimulus enables multiple cell liver repopulation by donor hepatocytes, LSECs, and bile duct cells.
目的
研究辐照（IR）和部分肝切除术（PH）是否可以为非实质细胞（NPC）移植准备宿主肝脏。
方法
用外照射 IR（25 Gy）预处理二肽基肽酶 IV（DPPIV）缺陷大鼠肝脏，将其右三叶的三分之二进行照射，然后对未照射的三叶进行三分之一 PH。将 DPPIV 阳性的肝细胞（NPC 制剂富含肝窦内皮细胞（LSECs）和肝细胞）通过脾脏移植到受体肝脏中。在移植后长达 16 周的时间内，研究供体细胞植入和生长的程度和质量。
结果
宿主肝脏染色显示出 3 种不同的再殖类型。可以检测到具有 DPPIV 管腔表达的供体衍生肝细胞的明确簇，这些肝细胞要么紧邻，要么位于表达内皮标志物血小板内皮细胞黏附分子的供体细胞大片区之间。第三种类型由 DPPIV 阳性的胆管样结构组成，这些结构与胆管上皮细胞 CD49f 共定位。
结论
肝 IR 和 PH 作为预处理刺激物，可使供体肝细胞、LSECs 和胆管细胞进行多细胞肝再殖。
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