Siddique Abu Baker, Moniruzzaman M, Ali Sobur, Dewan Md Nayem, Islam Mohammad Rafiqul, Islam Md Shafiqul, Amin Mohammed Badrul, Mondal Dinesh, Parvez Anowar Khasru, Mahmud Zahid Hayat
Laboratory of Environmental Health, Laboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
Department of Microbiology, Jahangirnagar University, Dhaka, Bangladesh.
Front Microbiol. 2021 Mar 8;12:635539. doi: 10.3389/fmicb.2021.635539. eCollection 2021.
is a major foodborne pathogen responsible for significant economic losses in aquaculture and a threat to human health. Here, we explored the incidence, virulence potential, and diversity of isolates from aquaculture farms in Bangladesh. We examined a total of 216 water, sediment, (tilapia), (rui), and (shrimp) samples from the aquaculture system where 60.2% (130/216) samples were positive for . Furthermore, we identified 323 strains from contaminated samples, 17 of which were found positive for , a virulence gene. Four isolates out of the 17 obtained were able to accumulate fluid in the rabbit ileal loop assay. The correlation between the contamination of and environmental factors was determined by Pearson correlation. The temperature and salinity were significantly correlated (positive) with the incidence of . Most of the pathogenic isolates (94.1%) were found resistant to ampicillin and amoxicillin. O8: KUT was the predominant serotype of the potentially pathogenic isolates. ERIC-PCR reveals genetic variation and relatedness among the pathogenic isolates. Therefore, this region-specific study establishes the incidence of potential infection with from the consumption of tilapia, rui, and shrimp raised in farms in Satkhira, Bangladesh, and the basis for developing strategies to reduce the risk for diseases and economic burden.
是一种主要的食源性病原体，在水产养殖中造成重大经济损失，并对人类健康构成威胁。在此，我们探究了孟加拉国水产养殖场分离株的发生率、毒力潜力和多样性。我们检查了来自水产养殖系统的总共216份水、沉积物、罗非鱼、鲤鱼和虾样本，其中60.2%（130/216）的样本检测呈阳性。此外，我们从受污染样本中鉴定出323株菌株，其中17株被发现毒力基因呈阳性。在获得的17株分离株中，有4株在兔回肠袢试验中能够积液。通过Pearson相关性确定了与环境因素之间的污染相关性。温度和盐度与的发生率显著相关（呈正相关）。大多数致病分离株（94.1%）对氨苄青霉素和阿莫西林耐药。O8:KUT是潜在致病分离株的主要血清型。ERIC-PCR揭示了致病分离株之间的遗传变异和相关性。因此，这项区域特异性研究确定了食用孟加拉国萨特希拉养殖场养殖的罗非鱼、鲤鱼和虾而感染的潜在发生率，以及制定降低疾病风险和经济负担策略的依据。
