Drug Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
Int J Radiat Biol. 2010 Dec;86(12):1070-8. doi: 10.3109/09553002.2010.501844. Epub 2010 Aug 16.
To investigate the possible role of vitamin E, coenzyme Q10 and rutin in ameliorating the biochemical changes in brain and serum induced by cerebral ischemia/reperfusion (I/R) in whole body γ-irradiated rats.
Cerebral ischemia was induced in male Wistar rats (either irradiated or non-irradiated) followed by reperfusion.
I/R increased brain content of malondialdehyde (MDA) and depleted its glutathione (GSH) content with a compensatory elevation in cytosolic activities of glutathione peroxidase (GPx) and glutathione reductase (GR) enzymes. It also raised brain cytosolic lactate dehydrogenase (LDH) activity and calcium (Ca(2+)) level. Furthermore, I/R provoked an inflammatory response reflected by an increment in serum levels of the proinflammatory cytokines tumour necrosis factor-α (TNF-α) and interlukin-1β (IL-1β). Moreover, induction of I/R in irradiated rats resulted in a further increase in brain oxidative stress and cytosolic LDH activity, disturbed brain Ca(2+) homeostasis and exaggerated the inflammatory reaction. During irradiation, administration of each of vitamin E, coenzyme Q10 (CoQ10) and rutin to irradiated rats before induction of I/R, alleviated the brain oxidative stress. Moreover, these antioxidants caused attenuation of the rise of the cytosolic activities of GPx and GR. A lowering effect of the cytosolic LDH activity and Ca(2+) level were caused by treatment with antioxidants. Each of vitamin E and rutin revealed an anti-inflammatory action of these antioxidants, while CoQ10 had no effect on serum levels of TNF-α and IL-1β.
These findings indicate that supplementation with either vitamin E, CoQ10 or rutin ameliorated most of the biochemical changes induced by I/R in irradiated rat brain and serum.
研究维生素 E、辅酶 Q10 和芦丁在改善全身 γ 射线照射大鼠脑和血清中脑缺血再灌注（I/R）引起的生化变化中的可能作用。
雄性 Wistar 大鼠（照射或未照射）诱导脑缺血，再灌注。
I/R 增加了脑丙二醛（MDA）含量，耗竭了谷胱甘肽（GSH）含量，同时细胞溶质谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的活性升高。它还提高了脑细胞溶质乳酸脱氢酶（LDH）活性和钙（Ca（2+））水平。此外，I/R 引发了炎症反应，血清中促炎细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平升高。此外，在照射大鼠中诱导 I/R 会导致脑氧化应激和细胞溶质 LDH 活性进一步增加，破坏脑 Ca（2+）稳态，并加剧炎症反应。在照射过程中，在诱导 I/R 之前，向照射大鼠给予维生素 E、辅酶 Q10（CoQ10）和芦丁中的每一种，都可以减轻脑氧化应激。此外，这些抗氧化剂降低了 GPx 和 GR 细胞溶质活性的升高。抗氧化剂还降低了细胞溶质 LDH 活性和 Ca（2+）水平。维生素 E 和芦丁均表现出抗氧化剂的抗炎作用，而 CoQ10 对 TNF-α和 IL-1β 的血清水平没有影响。
这些发现表明，补充维生素 E、CoQ10 或芦丁可改善照射大鼠脑和血清中由 I/R 引起的大多数生化变化。
