Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Viral Immunol. 2010 Aug;23(4):403-13. doi: 10.1089/vim.2010.0025.
Porcine reproductive and respiratory syndrome virus (PRRSV) can induce severe reproductive failure in sows, and is involved in the porcine respiratory disease complex. The glycoprotein GP4 of the European prototype PRRSV strain Lelystad virus (LV) contains a linear neutralizing epitope that is located in a highly variable region. The current study aimed to evaluate the antibody response against this and other epitopes on GP4 to infection of pigs with European-type PRRSV. It was shown that three virus strains, differing in the region that corresponds to the neutralizing epitope on GP4 of LV, strongly induce antibodies against this area. Antibodies against the epitopes of the different virus strains were purified from polyclonal swine sera, and used in virus-neutralization tests on primary alveolar macrophages. This revealed that antibodies against the variable region in GP4 of different virus strains are able to neutralize infection with homologous but not heterologous virus strains.
猪繁殖与呼吸综合征病毒（PRRSV）可引起母猪严重繁殖障碍，并与猪呼吸道疾病综合征有关。欧洲原型 PRRSV 株 Lelystad 病毒（LV）的糖蛋白 GP4 含有线性中和表位，位于高度可变区域。本研究旨在评估针对该中和表位和 GP4 上其他表位的抗体反应，以感染欧洲型 PRRSV 的猪。结果表明，三种病毒株在与 LV 的 GP4 上中和表位相对应的区域存在差异，强烈诱导针对该区域的抗体。从多克隆猪血清中纯化针对不同病毒株表位的抗体，并用于原代肺泡巨噬细胞的病毒中和试验。结果表明，针对不同病毒株 GP4 可变区的抗体能够中和同源但不能中和异源病毒株的感染。
