Adnyane I K M, Zuki A B, Noordin M M, Agungpriyono S
Department of Anatomy, Physiology and Pharmacology, Bogor Agricultural University (IPB), Indonesia.
Anat Histol Embryol. 2011 Oct;40(5):365-74. doi: 10.1111/j.1439-0264.2011.01081.x. Epub 2011 May 5.
The morphology, distribution and relative frequencies of endocrine cells in the gastrointestinal tract of the barking deer (Muntiacus muntjak) were investigated. The immunohistochemical method employed used seven types of antisera against chromogranin, serotonin, gastrin, cholecystokinin, somatostatin, glucagon and insulin. Chromogranin and serotonin immunoreactive (IR) cells were found throughout the gastrointestinal tract. Conversely, gastrin-IR cells were only found in the oxyntic gland, pyloric gland and duodenum, while cholecystokinin-IR and somatostatin-IR cells were detected in the oxyntic gland, pyloric gland and small intestines. Somatostatin-IR cells were also seen in the caecum. Glucagon-IR cells were found in all parts of the gastrointestinal tract apart from the colon and rectum. No insulin-IR cells were found in the gastrointestinal tract of this species. The cells in the small intestine were generally spindle shaped with long cytoplasmic processes ending in the lumen (open type), while in the stomach and large intestine, they were occasionally round or spherical in shape (closed type). An uncommon distribution pattern of endocrine cells in the gastrointestinal tract of the barking deer was noted for cholecystokinin- and glucagon-IR cells.
对赤麂胃肠道内分泌细胞的形态、分布及相对频率进行了研究。采用免疫组织化学方法，使用了七种针对嗜铬粒蛋白、5-羟色胺、胃泌素、胆囊收缩素、生长抑素、胰高血糖素和胰岛素的抗血清。在整个胃肠道均发现了嗜铬粒蛋白和5-羟色胺免疫反应（IR）细胞。相反，胃泌素IR细胞仅在胃底腺、幽门腺和十二指肠中发现，而胆囊收缩素IR细胞和生长抑素IR细胞则在胃底腺、幽门腺和小肠中检测到。在盲肠中也可见到生长抑素IR细胞。除结肠和直肠外，在胃肠道各部位均发现了胰高血糖素IR细胞。在该物种的胃肠道中未发现胰岛素IR细胞。小肠中的细胞通常呈纺锤形，有长的细胞质突起伸向管腔（开放型），而在胃和大肠中，它们偶尔呈圆形或球形（封闭型）。赤麂胃肠道中胆囊收缩素和胰高血糖素IR细胞的分布模式较为罕见。
