Canli Ozden, Erdal Serkan, Taskin Mesut, Kurbanoglu Esabi Basaran
Department of Biology, Science Faculty, Ataturk University, Erzurum, Turkey.
Toxicol Ind Health. 2011 Feb;27(1):35-9. doi: 10.1177/0748233710380219. Epub 2010 Aug 16.
Invertase is an important enzyme used in many fields especially in food industry to produce fructose syrups. The current study focused on increasing invertase production by exposing Rhodotorula glutinis to extremely low magnetic fields (ELMF; 0 and 7 mT). For this purpose, the microorganism was allowed to grow in normal magnetic field and ELMF for 72 hours at the same temperature (24 ± 2°C). The fermentation was carried out in submerged culture for 120 hours. The results showed that invertase production is strongly dependent on the growth conditions of the microorganism. Both of the different magnetic fields applied to R. glutinis increased invertase production ranged from 48%-67% when compared with the control. On the other hand, ELMF treatment increased biomass formation about 14%-28% when compared with the control. As a result, magnetic field treatment could effectively be used in the production of invertase by R. glutinis.
转化酶是一种重要的酶，在许多领域尤其是食品工业中用于生产果糖糖浆。当前的研究聚焦于通过将粘红酵母暴露于极低磁场（ELMF；0和7毫特斯拉）来提高转化酶产量。为此，使该微生物在相同温度（24±2°C）下于正常磁场和极低磁场中生长72小时。发酵在深层培养中进行120小时。结果表明，转化酶产量强烈依赖于微生物的生长条件。与对照相比，施加于粘红酵母的两种不同磁场均使转化酶产量提高了48% - 67%。另一方面，与对照相比，极低磁场处理使生物量形成增加了约14% - 28%。因此，磁场处理可有效地用于粘红酵母生产转化酶。
