School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, United Kingdom.
Proc Natl Acad Sci U S A. 2010 Aug 31;107(35):15329-34. doi: 10.1073/pnas.1006447107. Epub 2010 Aug 16.
The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with high efficiency (trans-ligation). In an alternative strategy, intrastrand click ligation was employed to produce a functional hammerhead ribozyme containing a novel nucleic acid backbone mimic at the catalytic site (cis-ligation). The ability to synthesize long RNA strands by a combination of solid-phase synthesis and click ligation is an important addition to RNA chemistry. It is compatible with a plethora of site-specific modifications and is applicable to the synthesis of many biologically important RNA molecules.
铜催化的叠氮-炔环加成反应（点击化学）已被用于与固相合成相结合，以生产大约 100 个核苷酸长的催化活性发夹核酶。通过相邻碱基的跨链连接，可以有效地将预合成的 RNA 链进行共价连接（反式连接）。在另一种策略中，采用链内点击连接来产生具有新颖核酸骨架模拟物的功能锤头核酶（顺式连接）。通过固相合成和点击连接相结合来合成长的 RNA 链的能力是 RNA 化学的一个重要补充。它与大量的位点特异性修饰兼容，并且适用于许多生物重要的 RNA 分子的合成。
