Section for Microbiology and Immunology, The Gade Institute, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway.
Caries Res. 2010;44(4):402-7. doi: 10.1159/000316664. Epub 2010 Aug 12.
Streptococcus mutans and Streptococcus sobrinus are among the most commonly isolated bacterial species implicated as etiological agents of dental caries. Details of the composition of the oral microflora related to dental caries should aid in assessing the prevalence and risk of disease at an individual level. The aim of the present study was to determine the presence and relative amounts of S. mutans and S. sobrinus in the saliva samples obtained from schoolchildren in Khartoum State, the Sudan, and to study the association of the amounts of S. mutans and S. sobrinus with caries experience, socioeconomic status and sugar-sweetened snacks in this population. 140 samples, 30 of which were from individuals with caries experience, were examined using quantitative real-time polymerase chain reaction (qRT-PCR) with specific oligonucleotide primers. The mean ratio of fold differences of S. mutans to S. sobrinus was 0.77 (SD 5.4) and 2.29 (SD 6.0) for samples obtained from caries-free and caries-active individuals, respectively. This suggested that the proportion of S. sobrinus was higher than S.mutans in the caries-active group when compared to the caries-free group. An association was found between children with caries-active lesions and the frequent consumption of sticky desserts and higher socioeconomic status. S. sobrinus seems to be associated with caries experience in the studied population. A proposal of caries screening programs designed to test for S. sobrinus in this population may be developed.
变形链球菌和远缘链球菌是最常被分离出来的与龋齿有关的细菌种属，与龋齿有关的口腔微生物组成的详细信息应有助于评估个体的疾病流行程度和发病风险。本研究旨在确定苏丹喀土穆州儿童唾液样本中变形链球菌和远缘链球菌的存在情况和相对数量，并研究该人群中变形链球菌和远缘链球菌的数量与龋齿患病情况、社会经济状况和含糖零食之间的关系。使用特异性寡核苷酸引物的实时聚合酶链反应（qRT-PCR）对 140 个样本进行了检测，其中 30 个样本来自有龋齿患病经历的个体。无龋和有龋个体样本中变形链球菌与远缘链球菌的比值平均差异倍数分别为 0.77（SD 5.4）和 2.29（SD 6.0）。这表明与无龋组相比，有龋组中远缘链球菌的比例高于变形链球菌。有龋损病变的儿童与经常食用粘性甜点和较高社会经济地位之间存在关联。远缘链球菌似乎与研究人群中的龋齿患病情况有关。可能会针对该人群制定旨在检测远缘链球菌的龋齿筛查计划。
