Shafaie Ebrahim, Badri Zahra, Salehiniya Hamid, Abbaszadeh Hamid
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
Heliyon. 2024 Feb 6;10(3):e25663. doi: 10.1016/j.heliyon.2024.e25663. eCollection 2024 Feb 15.
Dental plaque bacteria, including (), play a role in the pathogenesis of the dental caries. There are conflicting results regarding the association of salivary level and dental caries susceptibility. Our aim was to compare salivary levels in colony-forming units (CFU) between children with active caries and caries-free children in Birjand, Iran.
This case-control study included 61 six-year-old children referred to health centers in Birjand city, Iran, in 2022. The children were divided into two groups: case (dmft/DMFT>0 with active caries) (including 31 children) and control (dmft/DMFT = 0 [caries-free]) (including 30 children). Demographic information and dental history were recorded. Oral examinations were also performed by the dentist. Unstimulated saliva samples were collected from children. The number of salivary colonies was determined using the microbial culture and confirmed using the polymerase chain reaction (PCR). The data were analyzed using Chi-square and T-tests at a significance level of p < 0.05.
The mean number of colonies was 126.24 ± 92.78 CFU/ml and 92.38 ± 75.34 CFU/ml in case and control groups, respectively. No significant difference was found in salivary levels between case and control groups (P = 0.125). No significant association was observed between caries experience with gender (P = 0.363), type of school (public/private) (P = 0.296), receiving oral health education (P = 0.072) and frequency of tooth brushing (P = 0.935). The mean gingival index (P = 0.001) and plaque index (P = 0.025) in case group were significantly higher than control group.
There is no significant difference in salivary levels between caries-active and caries-free children in Birjandi children.
包括（）在内的牙菌斑细菌在龋齿的发病机制中起作用。关于唾液水平与龋齿易感性之间的关联，存在相互矛盾的结果。我们的目的是比较伊朗比尔詹德地区患活动性龋齿儿童和无龋齿儿童唾液中以菌落形成单位（CFU）计的水平。
这项病例对照研究纳入了2022年转诊至伊朗比尔詹德市健康中心的61名6岁儿童。这些儿童被分为两组：病例组（dmft/DMFT>0且患有活动性龋齿）（包括31名儿童）和对照组（dmft/DMFT = 0[无龋齿]）（包括30名儿童）。记录人口统计学信息和牙科病史。牙医也进行了口腔检查。从儿童中收集未刺激的唾液样本。使用微生物培养法测定唾液菌落数量，并通过聚合酶链反应（PCR）进行确认。数据采用卡方检验和t检验进行分析，显著性水平为p < 0.05。
病例组和对照组唾液菌落的平均数量分别为126.24±92.78 CFU/ml和92.38±75.34 CFU/ml。病例组和对照组之间的唾液水平无显著差异（P = 0.125）。龋齿经历与性别（P = 0.363）、学校类型（公立/私立）（P = 0.296）、接受口腔健康教育（P = 0.072）和刷牙频率（P = 0.935）之间均未观察到显著关联。病例组的平均牙龈指数（P = 0.001）和菌斑指数（P = 0.025）显著高于对照组。
在比尔詹德儿童中，患龋齿儿童和无龋齿儿童的唾液水平无显著差异。
