Kilaru Aruna, Isaac Giorgis, Tamura Pamela, Baxter David, Duncan Scott R, Venables Barney J, Welti Ruth, Koulen Peter, Chapman Kent D
Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203-5017, USA.
Lipids. 2010 Sep;45(9):863-75. doi: 10.1007/s11745-010-3457-5. Epub 2010 Aug 17.
N-Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon (N-acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase (FAAH (-/-)) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of FAAH (-/-) mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.
N-酰基乙醇胺（NAE）是脂肪酸衍生物，其中一些在哺乳动物中作为内源性大麻素发挥作用。NAE代谢涉及常见的（磷脂酰乙醇胺，PE）和不常见的（N-酰基磷脂酰乙醇胺，NAPE）膜磷脂。在此，我们已鉴定并定量了小鼠脑和心脏组织中NAE/内源性大麻素途径中的一百多种代谢物，包括许多先前未报道的NAPE分子种类。我们发现，缺乏脂肪酸酰胺水解酶（FAAH（-/-））的小鼠脑组织中，除了NAE升高外，PE和NAPE分子种类也升高，这表明FAAH活性参与了该途径的整体调节。在FAAH（-/-）小鼠的心脏组织中未观察到脑内NAE途径的这种扰动，这表明NAE途径的代谢调节在这两个器官中有所不同，并且分解代谢NAE的代谢酶很可能分布和/或调节存在差异。像本文所展示的靶向脂质组学分析，将继续为细胞脂质信号网络提供重要见解。
