Institute of Photonics and Electronics AS CR, Chaberská 57, Prague 8, Czech Republic.
Anal Bioanal Chem. 2010 Nov;398(5):1963-6. doi: 10.1007/s00216-010-4067-z. Epub 2010 Aug 12.
We present a compact surface plasmon resonance (SPR) biosensor for the detection of bisphenol A (BpA), an endocrine-disrupting chemical. The biosensor is based on an SPR sensor platform (SPRCD) and the binding inhibition detection format. The detection of BpA in PBS and wastewater was performed at concentrations ranging from 0.05 to 1,000 ng/ml. The limit of detection for BpA in PBS and wastewater was estimated to be 0.08 and 0.14 ng/ml, respectively. It was also demonstrated that the biosensor can be regenerated for repeated use. Results achieved with the SPR biosensor are compared with those obtained using ELISA and HPLC methods.
我们提出了一种紧凑的表面等离子体共振（SPR）生物传感器，用于检测双酚 A（BpA），这是一种内分泌干扰化学物质。该生物传感器基于 SPR 传感器平台（SPRCD）和结合抑制检测格式。在 PBS 和废水中，BpA 的检测浓度范围为 0.05 至 1000ng/ml。在 PBS 和废水中，BpA 的检测限估计分别为 0.08 和 0.14ng/ml。还证明了该生物传感器可以再生，以便重复使用。将 SPR 生物传感器获得的结果与使用 ELISA 和 HPLC 方法获得的结果进行了比较。
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