School of Environment and Natural Resources, Renmin University of China, Beijing, China.
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Biosens Bioelectron. 2014 May 15;55:19-25. doi: 10.1016/j.bios.2013.11.069. Epub 2013 Dec 4.
Bisphenol A (BPA), a xenoestrogenic endocrine-disrupting chemical, is used in many consumer products worldwide and is widely detected in the environment and in food. Combining the advantages of evanescent wave fiber optic sensor and microfluidic technology, an all-fiber optofluidics-based bioassay platform (AFOB) was developed for the rapid immunoassay and assessment of BPA. The captured molecular BPA-bovine serum albumin was covalently immobilized on the surface of the fiber optic sensor. A mixture of different concentrations of BPA and a certain concentration of fluorescence-labeled anti-BPA monoclonal antibodies after pre-reaction was introduced to the optofluidic cell. A higher concentration of BPA reduced the fluorescence-labeled antibodies bound to the sensor surface and thus reduced fluorescence signals. Under optimal conditions, the BPA quantified as 0.5-100 μg/L, with a detection limit of 0.06 μg/L. The high selectivity of the sensor was evaluated in terms of its response to several potentially interfering chemicals. The potential interference of an environmental sample matrix was assessed by spiked samples, and the recovery of BPA ranged from 90% to 120% with relative standard deviation values of <9.1%. The AFOB and high-performance liquid chromatography had a desired correlation (R(2)=0.9958). The sensing platform was successfully used to assess BPA leaching from polycarbonate bottles at 45 °C and 80 °C, indicating that more BPA was substantially leached at elevated temperature and extend time. Thus, the developed sensing strategy can be an alternative method to rapidly analyze and assess the migration mechanism and fate of BPA or other pollutants.
双酚 A（BPA）是一种外源性雌激素类内分泌干扰化学物质，广泛应用于世界范围内的许多消费品中，并广泛存在于环境和食品中。本研究结合了倏逝波光纤传感器和微流控技术的优点，开发了一种基于全光纤光流控的生物分析平台（AFOB），用于快速免疫分析和评估双酚 A。捕获的分子 BPA-牛血清白蛋白被共价固定在光纤传感器的表面上。将不同浓度的 BPA 混合物和一定浓度的荧光标记抗 BPA 单克隆抗体在预反应后引入光流控池。较高浓度的 BPA 减少了与传感器表面结合的荧光标记抗体，从而降低了荧光信号。在最佳条件下，BPA 的定量范围为 0.5-100 μg/L，检测限为 0.06 μg/L。该传感器的高选择性通过其对几种潜在干扰化学物质的响应来评估。通过加标样品评估环境样品基质的潜在干扰，BPA 的回收率在 90%-120%之间，相对标准偏差值<9.1%。AFOB 与高效液相色谱法具有良好的相关性（R(2)=0.9958）。该传感平台成功用于评估聚碳酸酯瓶在 45°C 和 80°C 下的 BPA 浸出情况，表明在高温和延长时间下，BPA 大量浸出。因此，所开发的传感策略可以作为一种替代方法，用于快速分析和评估 BPA 或其他污染物的迁移机制和归宿。
