Departamento de Edafología y Química Agrícola. Fac. Farmacia, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Biodegradation. 2011 Jul;22(4):763-71. doi: 10.1007/s10532-010-9402-8. Epub 2010 Aug 17.
The application of biocides is a traditional method of controlling biodecay of outdoor cultural heritage. Chlorophyll degradation to phaeopigments is used to test the biocidal efficacy of the antimicrobial agents. In the present study, the usefulness of color measurements in estimating chlorophyll degradation was investigated. An aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc was chosen as test organism, comparing its different behaviour in both planktonic and biofilm mode of growth against the isothiazoline biocide Biotin T®. Changes in A(435 nm)/A(415 nm) and A(665 nm)/A(665a nm) and in the chlorophyll a and adenosine triphosphate (ATP) cell content were compared with the variations in the CIELAB color parameters (L*, a*, b*, C*(ab) and h(ab)). Our findings showed that both the phaeophytination indexes are useful in describing degradation of chlorophyl a to phaeopigments. Moreover, the CIELAB color parameters represented an effective tool in describing chlorophyll degradation. L* CIELAB parameter appeared to be the most informative parameter in describing the biocidal activity of Biotin T® against Nostoc sp. in both planktonic and biofilm mode of growth.
杀菌剂的应用是控制户外文化遗产生物降解的传统方法。叶绿素降解为脱镁叶绿素用于测试抗菌剂的杀菌功效。在本研究中，研究了颜色测量在估计叶绿素降解中的有用性。选择了一种气生石生蓝藻属 Nostoc 的蓝细菌作为测试生物，比较了其在浮游和生物膜生长模式下对异噻唑啉杀菌剂 Biotin T®的不同行为。将 A(435nm)/A(415nm)和 A(665nm)/A(665a nm)的变化与 CIELAB 颜色参数 (L*、a*、b*、C*(ab)和 h(ab))的变化进行了比较。我们的研究结果表明，两种脱镁叶绿素指数都可用于描述叶绿素 a 向脱镁叶绿素的降解。此外，CIELAB 颜色参数是描述 Biotin T®对 Nostoc sp. 在浮游和生物膜生长模式下的杀菌活性的有效工具。L*CIELAB 参数似乎是描述 Biotin T®对 Nostoc sp. 浮游和生物膜生长模式下的杀菌活性的最具信息量的参数。
