Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Universidad Antonoma de Queretaro, Santiago de Queretaro, Queretaro, Mexico 36821.
J Agric Food Chem. 2010 Sep 8;58(17):9542-8. doi: 10.1021/jf102590p.
Total polyphenolic and anthocyanin- and proanthocyanidin-rich fractions from wild blackberry genotypes (WB-3, WB-7, WB-10, and WB-11), a domesticated noncommercial breeding line (UM-601), and a commercial cultivar (Tupy) were evaluated for inhibition of pro-inflammatory responses [nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and prostaglandin E2 (PGE2)] in RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). At 50 microM [cyanidin-3-O-glucoside (C3G) or catechin equivalent], most fractions significantly (P<0.05) inhibited all markers. The anthocyanin-rich fraction from WB-10 and the proanthocyanidin-rich fraction from UM-601 exhibited the highest NO inhibitory activities (IC50=16.1 and 15.1 microM, respectively). Proanthocyanidin-rich fractions from the wild WB-10 showed the highest inhibition of iNOS expression (IC50=8.3 microM). Polyphenolic-rich fractions from WB-7 and UM-601 were potent inhibitors of COX-2 expression (IC50=19.1 and 19.3 microM C3G equivalent, respectively). For most of the extracts, antioxidant capacity was significantly correlated with NO inhibition. Wild genotypes of Mexican blackberries, as rich sources of polyphenolics that have both antioxidant and anti-inflammatory properties, showed particular promise for inclusion in plant improvement programs designed to develop new varieties with nutraceutical potential.
总多酚和富含花色苷和原花青素的野生黑莓基因型（WB-3、WB-7、WB-10 和 WB-11）、一个驯化的非商业育种系（UM-601）和一个商业品种（Tupy）的分数，评估了对脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞促炎反应的抑制作用[一氧化氮（NO）产生、诱导型一氧化氮合酶（iNOS）表达、环氧化酶-2（COX-2）表达和前列腺素 E2（PGE2）]。在 50 μM [矢车菊素-3-O-葡萄糖苷（C3G）或儿茶素当量]时，大多数分数显着（P<0.05）抑制了所有标志物。WB-10 的花色苷丰富部分和 UM-601 的原花青素丰富部分表现出最高的 NO 抑制活性（IC50=16.1 和 15.1 μM，分别）。来自野生 WB-10 的原花青素丰富部分显示出对 iNOS 表达的最高抑制作用（IC50=8.3 μM）。WB-7 和 UM-601 的多酚丰富部分是 COX-2 表达的有效抑制剂（IC50=19.1 和 19.3 μM C3G 当量，分别）。对于大多数提取物，抗氧化能力与 NO 抑制呈显著相关。作为具有抗氧化和抗炎特性的多酚的丰富来源的墨西哥黑莓野生基因型，特别有望被纳入旨在开发具有营养潜力的新品种的植物改良计划。
