Department of Plastic and Hand Surgery, University of Erlangen-Nürnberg, Medical Center, Krankenhausstrasse 12, Erlangen, Germany.
J Cell Mol Med. 2011 Jul;15(7):1564-71. doi: 10.1111/j.1582-4934.2010.01147.x.
Chronic wounds--as defined by the World Union of Wound Healing Societies (WUWHS)--are a considerable worldwide health care expense and impair quality of life. In order for chronic wounds to heal, these wounds must be transformed to a more acute state to begin the healing process. Topical negative pressure (TNP) with reticulated open cell foam (ROCF) is known to promote healing in certain types of chronic wounds. However, little is known about changes at the cellular or molecular level in wounds under various treatments, especially under the physical forces induced to tissue by TNP. In the current study, chronic wound samples were obtained during routine wound debridements prior to treatment and 7-12 days after initiating TNP with a continuous setting at -125 mmHg. Whole genome transcriptome microarray analyses were performed on samples to better understand how TNP with ROCF affects these types of wounds. It was found that more genes were expressed following TNP with ROCF as compared to before therapy and to normal, non-wounded tissue. In this study, we show that TNP with ROCF transforms the chronic wound from its inflammation (non-healing) state into more of a progressive, healing phenotype from a molecular point of view with expression of genes that are commonly associated with these terms.
慢性创面——根据世界伤口愈合学会联盟（WUWHS）的定义——是一个相当大的全球医疗保健费用，并损害生活质量。为了使慢性创面愈合，这些创面必须转化为更急性的状态，开始愈合过程。网孔泡沫状的局部负压（TNP）已被证实可促进某些类型的慢性创面愈合。然而，对于各种治疗方法下（特别是 TNP 对组织施加的物理力下）创面的细胞或分子水平变化，人们知之甚少。在本研究中，在开始用 -125mmHg 的持续负压进行 TNP 治疗之前和 7-12 天后，在常规创面清创过程中获得慢性创面样本。对样本进行全基因组转录组微阵列分析，以更好地了解 ROCF 的 TNP 如何影响这些类型的创面。结果发现，与治疗前相比，TNP 后 ROCF 表达的基因更多，与正常非创伤组织相比也是如此。在这项研究中，我们从分子角度表明，TNP 与 ROCF 将慢性创面从炎症（不愈合）状态转化为更具进展性的愈合表型，表达的基因通常与这些术语相关。
