Núcleo de Pós-Graduação e Pesquisa, Hospital Santa Casa de Belo Horizonte, Minas Gerais, Brasil.
Oxid Med Cell Longev. 2009 Nov-Dec;2(5):317-21. doi: 10.4161/oxim.2.5.9657.
Peripheral blood mononuclear cells (PBMNC) from patients with type 2 diabetes (DM2) have generated higher levels of reactive oxygen species (ROS) that were higher than those in cells from healthy individuals. In the presence of a cAMP-elevating agent, ROS production was significantly activated in PBMNC from DM2 patients but it was inhibited in cells from healthy subjects. Higher levels of IL-6 has been detected in the supernatant of PBMNC cultures from DM2 patients in comparison with healthy controls. When cells were cultured in the presence of a cAMP-elevating agent, the level of IL-6 decreased has by 46% in the supernatant of PBMNC from DM2 patients but it remained unaltered in controls. No correlations between ROS and IL-6 levels in PBMNC from DM2 patients or controls have been observed. Secretions of IL-4 or IFNgamma by PBMNC from patients or controls have not been affected by the elevation of cAMP. cAMP elevating agents have activated the production of harmful reactive oxidant down modulated IL-6 secretion by these cells from DM2 patients, suggesting an alteration in the metabolic response possibly due to hyperglicemia. The results suggest that cAMP may play an important role in the pathogenesis of diabetes.
外周血单个核细胞（PBMNC）来自 2 型糖尿病（DM2）患者，产生的活性氧（ROS）水平高于健康个体的细胞。在 cAMP 升高剂存在的情况下，DM2 患者的 PBMNC 中 ROS 的产生明显被激活，但健康受试者的细胞则受到抑制。与健康对照组相比，来自 DM2 患者的 PBMNC 培养物上清液中检测到更高水平的白细胞介素 6（IL-6）。当细胞在 cAMP 升高剂存在下培养时，DM2 患者 PBMNC 上清液中的 IL-6 水平下降了 46%，而对照组则没有变化。在 DM2 患者或对照组的 PBMNC 中，ROS 和 IL-6 水平之间没有观察到相关性。cAMP 升高剂并未影响患者或对照者的 PBMNC 分泌 IL-4 或 IFNgamma。cAMP 升高剂激活了有害活性氧化剂的产生，下调了这些来自 DM2 患者的细胞的 IL-6 分泌，表明代谢反应的改变可能是由于高血糖。这些结果表明，cAMP 可能在糖尿病的发病机制中发挥重要作用。
