Clymer B D, Devore T B, Jagadeesh J, Tomei L D
Appl Opt. 1991 Dec 1;30(34):5056-62. doi: 10.1364/AO.30.005056.
We present theory and experimental data on the minimum detectable feature size and spatial resolution for a scanning-laser microscope system that uses bulk photodetection. In the analysis, interactions of laser photons with an object are given a probability function that varies with the position within an object. Typical interactions that can be measured with such a scanning device include photon absorption (densitometry), scattering, and photofluorescence. Because bulk photodetection is used, image resolution is a function of laser-probe spot size and recording precision. We present data from simulations that predict a minimum separation between feature centers of approximately 1.56 times the half-width of the laser spot. Experimental verification by scans of U.S. Air Force test targets confirms this theory.
我们展示了关于使用体光电探测的扫描激光显微镜系统的最小可检测特征尺寸和空间分辨率的理论及实验数据。在分析中，激光光子与物体的相互作用被赋予一个随物体内位置变化的概率函数。能用这种扫描装置测量的典型相互作用包括光子吸收（密度测定法）、散射和光致荧光。由于使用了体光电探测，图像分辨率是激光探测光斑尺寸和记录精度的函数。我们展示了模拟数据，该数据预测特征中心之间的最小间距约为激光光斑半高宽的1.56倍。通过对美国空军测试靶标的扫描进行的实验验证证实了这一理论。
