使用非线性上转换纳米粒子可提高荧光漫射成像的空间分辨率。

Use of nonlinear upconverting nanoparticles provides increased spatial resolution in fluorescence diffuse imaging.

机构信息

Department of Physics, Lund University, P.O. Box 118, S-221 00 Lund, Sweden.

出版信息

Opt Lett. 2010 Aug 15;35(16):2789-91. doi: 10.1364/OL.35.002789.

DOI:10.1364/OL.35.002789

PMID:20717458

Abstract

Fluorescence diffuse imaging (FDI) suffers from limited spatial resolution. In this Letter, we report a scanning imaging approach to increase the resolution of FDI using nonlinear fluorophores. The resolution of a linear fluorophore was compared with nonlinear upconverting nanoparticles (NaYF(4):Yb(3+)/Tm(3+)) in a tissue phantom. A resolution improvement of a factor of 1.3 was found experimentally. Simulations suggested a maximum resolution improvement of a factor of 1.45. Usage of nonlinear fluorophores is a promising method for increasing the spatial resolution in FDI.

摘要

荧光漫射成像（FDI）受到空间分辨率的限制。在这封信中，我们报告了一种扫描成像方法，使用非线性荧光团来提高 FDI 的分辨率。我们在组织体模中比较了线性荧光团和非线性上转换纳米粒子（NaYF(4):Yb(3+)/Tm(3+)）的分辨率。实验发现分辨率提高了 1.3 倍。模拟表明最大分辨率可提高 1.45 倍。使用非线性荧光团是提高 FDI 空间分辨率的一种很有前途的方法。