Department of Analytical Chemistry, Stockholm University, 10691 Stockholm, Sweden.
Anal Bioanal Chem. 2011 Jan;399(1):191-5. doi: 10.1007/s00216-010-4092-y. Epub 2010 Aug 19.
Downscaled analytical tools for sample preparation have offered benefits such as higher throughput, easier automation and lower sample/reagent consumption. Microfluidic electrocapture, which is a newly developed sample preparation/manipulation system, uses an electric field to trap and separate charged species without using any solid sorbent. The feasibility of using microfluidic electrocapture is reported for separation, clean-up, concentration, microreactions and complexation studies of proteins, peptides and other biologically important biomolecules. The instrumentation and applications of microfluidic electrocapture are reviewed and an overview is provided of future perspectives offered by the current and envisaged platforms.
微流控电动捕获是一种新兴的样品制备/操作系统，它利用电场捕获和分离带电物质，而无需使用任何固相吸附剂。本文报道了微流控电动捕获在蛋白质、肽和其他重要生物分子的分离、净化、浓缩、微反应和络合研究中的应用。本文综述了微流控电动捕获的仪器和应用，并对当前和预期的平台所提供的未来前景进行了概述。
