Centre for Intelligent Systems Research, Deakin University, Pignonds Road, Waurn Ponds, VIC 3217, Australia.
Biosens Bioelectron. 2011 Jan 15;26(5):1800-14. doi: 10.1016/j.bios.2010.09.022. Epub 2010 Sep 17.
Dielectrophoresis, the induced motion of polarisable particles in a nonuniform electric field, has been proven as a versatile mechanism to transport, accumulate, separate and characterise micro/nano scale bioparticles in microfluidic systems. The integration of DEP systems into the microfluidics enables the inexpensive, fast, highly sensitive, highly selective and label-free detection and analysis of target bioparticles. This review provides an in-depth overview of state-of-the-art dielectrophoretic (DEP) platforms integrated into microfluidics aimed towards different biomedical applications. It classifies the current DEP systems in terms of different microelectrode configurations and operating strategies devised to generate and employ DEP forces in such processes, and compares the features of each approach. Finally, it suggests the future trends and potential applications of DEP systems in single cell analysis, stem cell research, establishing novel devices, and realising fully DEP-activated lab-on-a-chip systems.
介电泳，即各向异性颗粒在非均匀电场中的诱导运动，已被证明是一种在微流控系统中输送、聚集、分离和表征微/纳米级生物颗粒的多功能机制。DEP 系统与微流控技术的结合，实现了对目标生物颗粒的廉价、快速、高灵敏度、高选择性和无标记检测与分析。本综述深入概述了集成到微流控中的最先进的介电泳（DEP）平台，这些平台针对不同的生物医学应用。它根据不同的微电极配置和操作策略对当前的 DEP 系统进行分类，这些策略旨在产生和利用此类过程中的 DEP 力，并比较每种方法的特点。最后，它提出了 DEP 系统在单细胞分析、干细胞研究、新型器件开发以及实现完全 DEP 激活的微流控芯片系统中的未来趋势和潜在应用。
