Dr. Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA.
Cancer Metastasis Rev. 2010 Sep;29(3):529-42. doi: 10.1007/s10555-010-9244-1.
Consumption of a plant-based diet has been associated with prevention of the development and progression of cancer. We have developed strategies to inhibit cancer development and its spread by targeting common mechanisms used by all types of cancer cells that decrease stability and integrity of connective tissue. Strengthening of collagen and connective tissue can be achieved naturally through the synergistic effects of selected nutrients, such as lysine, proline, ascorbic acid and green tea extract (NM). This micronutrient mixture has exhibited a potent anticancer activity in vivo and in vitro in a few dozen cancer cell lines. Its anti-cancer effects include inhibition of metastasis, tumor growth, matrix metalloproteinase (MMP) secretion, invasion, angiogenesis, and cell growth as well as induction of apoptosis. Many cancers are often diagnosed at later stages, when metastasis has occurred, which standard treatment has been unable to control. Our studies on NM effects on hepatic and pulmonary metastasis demonstrated profound, significant suppression of metastasis in a murine model. Evaluation of effects of NM on xenografts in murine models demonstrated significant reduction in tumor size and tumor burden in all human cancer cell lines tested. In vitro studies demonstrated that NM was very effective in inhibition of cell proliferation (by MTT assay), MMP secretion (by gelatinase zymography), cell invasion (through Matrigel), cell migration (by scratch test), induction of apoptosis (by live green caspase) and induction of pro-apoptotic genes in many diverse cancer cell lines. Furthermore, in vivo and in vitro studies of effects of individual micronutrients compared to their specific combination demonstrated synergistic effects resulting in improved anticancer potency.
植物性饮食的消费与癌症的发生和发展的预防有关。我们已经开发了一些策略，通过针对所有类型的癌细胞都使用的共同机制来抑制癌症的发展及其扩散，这些共同机制会降低结缔组织的稳定性和完整性。通过选择的营养素（如赖氨酸、脯氨酸、抗坏血酸和绿茶提取物）的协同作用，可以自然地增强胶原蛋白和结缔组织的强度。这种微量营养素混合物在几十种癌细胞系的体内和体外都表现出了很强的抗癌活性。其抗癌作用包括抑制转移、肿瘤生长、基质金属蛋白酶（MMP）分泌、侵袭、血管生成和细胞生长以及诱导细胞凋亡。许多癌症通常在发生转移后被诊断出来，而标准治疗方法已经无法控制转移。我们对 NM 对肝和肺转移的影响的研究表明，它在小鼠模型中对转移有显著的抑制作用。对 NM 对小鼠模型中异种移植物的影响的评估表明，在所有测试的人类癌细胞系中，肿瘤大小和肿瘤负担都有显著减少。体外研究表明，NM 非常有效地抑制细胞增殖（通过 MTT 测定）、MMP 分泌（通过明胶酶谱图）、细胞侵袭（通过 Matrigel）、细胞迁移（通过划痕试验）、诱导细胞凋亡（通过活绿色 caspase）以及诱导许多不同的癌细胞系中的促凋亡基因。此外，与单独的微量营养素相比，对个体微量营养素及其特定组合的体内和体外研究表明，协同作用导致抗癌效力的提高。
