1 Dr. Rath Research Institute, Santa Clara, CA, USA.
Integr Cancer Ther. 2019 Jan-Dec;18:1534735419832365. doi: 10.1177/1534735419832365.
Tumor metastasis is a major cause for most cancer-related deaths. Melanoma is a serious cancer that metastasizes to other areas of the body, including the lungs, liver, brain, bones, or lymph nodes. Currently used cancer therapies are ineffective with a high degree of toxicity and patient mortality. Thus, any successful treatment for melanoma must target metastasis.
We studied the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, green tea extract, quercetin, and others, on the inhibition of melanoma growth and metastasis after inoculation of B16FO melanoma cells into the left kidney of female nude mice. Female athymic mice (n = 10) 8 to 10 weeks of age, were inoculated superficially in the left kidney with 5 × 10 B16FO melanoma cells in 100 µL of media. The right kidney was left untreated. After inoculation, the mice were randomly divided into 2 groups. The control group (n = 5) was fed a regular rodent chow diet, and the test group was given the same diet supplemented with 0.5% NM. The animals in control and the test groups were sacrificed 2 weeks later. Each animal's abdominal cavity was opened, and the kidneys, lungs, liver, and spleen were excised and examined for tumor growth and metastasis.
The kidneys in the control group weighed 25% to 30% more than those in the NM group due to colonization of B16FO melanoma cells. No metastasis to the liver or spleen was observed in either of the groups. However, severe lung metastasis was observed in the control group and mild to moderate metastasis was observed in the NM group.
These results show that the NM is effective in mitigating the growth of tumors in the kidney and metastases to the lung.
肿瘤转移是大多数癌症相关死亡的主要原因。黑色素瘤是一种严重的癌症，会转移到身体的其他部位，包括肺部、肝脏、大脑、骨骼或淋巴结。目前使用的癌症疗法效果不佳，且具有高度的毒性和患者死亡率。因此，任何成功的黑色素瘤治疗方法都必须针对转移。
我们研究了一种含有抗坏血酸、赖氨酸、脯氨酸、绿茶提取物、槲皮素等的新型营养混合物（NM）对 B16FO 黑色素瘤细胞接种到雌性裸鼠左肾后抑制黑色素瘤生长和转移的影响。8 至 10 周龄的雌性无胸腺小鼠（n = 10），在左肾浅层接种 5×10 B16FO 黑色素瘤细胞，每 100 µL 培养基中接种 100 µL。右肾未处理。接种后，将小鼠随机分为 2 组。对照组（n = 5）给予常规啮齿动物饲料，实验组给予相同饲料，补充 0.5% NM。对照组和实验组的动物在 2 周后处死。打开每个动物的腹腔，取出肾脏、肺、肝和脾，检查肿瘤生长和转移情况。
对照组的肾脏比 NM 组重 25%至 30%，因为有 B16FO 黑色素瘤细胞定植。两组均未观察到肝或脾转移。然而，对照组观察到严重的肺转移，NM 组观察到轻度至中度转移。
这些结果表明，NM 有效减轻了肾脏肿瘤的生长和肺转移。
