College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
Parasitol Res. 2010 Nov;107(6):1499-503. doi: 10.1007/s00436-010-2027-3. Epub 2010 Aug 18.
In the present study, the potential of RNA interference (RNAi) as a gene silencing tool and the resultant effects on Ascaris suum larval development was examined by targeting a gene (represented by the EST 06G09) specifically expressed in the infective larvae of A. suum. BALB/c mice were infected with RNAi-treated larvae. The results showed that the target gene was silenced after soaking for 72 h, and the survival rate of the RNAi-treated larvae was reduced by 17.25% (P<0.01). A significant difference (P<0.05) was detected in the numbers of larvae collected from the livers and lungs of infected mice 4 days after infection with untreated larvae (164.29 ± 21.51) and RNAi-treated larvae (71.43 ± 14.35). Significant differences (P<0.01) were also found in the body length and width between untreated larvae (480 ± 105.77 μm for length and 23.93 ± 3.72 μm for width) and RNAi-treated larvae (400.57 ± 71.31 μm for length and 20.20 ± 2.43 μm for width). These results show that the gene represented by EST 06G09 may play a role in the development of A. suum larvae.
在本研究中，通过针对猪蛔虫感染性幼虫中特异性表达的基因（由 EST 06G09 代表），研究了 RNA 干扰（RNAi）作为基因沉默工具的潜力及其对猪蛔虫幼虫发育的影响。用 RNAi 处理的幼虫感染 BALB/c 小鼠。结果表明，浸泡 72 小时后靶基因沉默，RNAi 处理幼虫的存活率降低 17.25%（P<0.01）。感染后 4 天，未处理幼虫（164.29±21.51）和 RNAi 处理幼虫（71.43±14.35）从感染小鼠的肝脏和肺部收集的幼虫数量存在显著差异（P<0.05）。未处理幼虫（体长 480±105.77μm，体宽 23.93±3.72μm）和 RNAi 处理幼虫（体长 400.57±71.31μm，体宽 20.20±2.43μm）之间的体长和体宽也存在显著差异（P<0.01）。这些结果表明，EST 06G09 代表的基因可能在猪蛔虫幼虫的发育中发挥作用。
