Knox David P, Geldhof Peter, Visser Aline, Britton Collette
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK.
Trends Parasitol. 2007 Mar;23(3):105-7. doi: 10.1016/j.pt.2007.01.007. Epub 2007 Feb 2.
RNA interference (RNAi) is widely used in Caenorhabditis elegans to identify gene function and has been adapted as a high-throughput screening method to identify genes involved in essential processes. The technique has been applied to parasitic nematodes with variable success and we believe that inconsistent outcomes preclude its use as a robust screen with which to identify potential control targets. In this article, key issues that require clarification are discussed, including the mode of delivery of double-stranded RNA to the parasite, the developmental stage targeted and, perhaps of most importance, whether the RNAi pathway (as defined by studies in C. elegans) is fully functional in some parasitic nematodes.
RNA干扰（RNAi）在秀丽隐杆线虫中被广泛用于鉴定基因功能，并已被改编为一种高通量筛选方法，以鉴定参与基本过程的基因。该技术已应用于寄生线虫，但成功率各不相同，我们认为结果不一致妨碍了它作为一种可靠的筛选方法来鉴定潜在的控制靶点。在本文中，讨论了需要澄清的关键问题，包括双链RNA传递到寄生虫的方式、靶向的发育阶段，以及也许最重要的是，RNAi途径（如秀丽隐杆线虫研究中所定义的）在某些寄生线虫中是否完全发挥作用。
