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曲古抑菌素 A 和 5-氮杂胞苷在玉米有丝分裂过程中均导致组蛋白 H4 乙酰化的整体增加和整体 DNA 和 H3K9 甲基化的减少。

Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize.

机构信息

Key laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.

出版信息

BMC Plant Biol. 2010 Aug 18;10:178. doi: 10.1186/1471-2229-10-178.

DOI:10.1186/1471-2229-10-178
PMID:20718950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3095308/
Abstract

BACKGROUND

Modifications of DNA and histones in various combinations are correlated with many cellular processes. In this study, we investigated the possible relationship between histone H4 tetraacetylation, DNA methylation and histone H3 dimethylation at lysine 9 during mitosis in maize root meristems.

RESULTS

Treatment with trichostatin A, which inhibits histone deacetylases, resulted in increased histone H4 acetylation accompanied by the decondensation of interphase chromatin and a decrease in both global H3K9 dimethylation and DNA methylation during mitosis in maize root tip cells. These observations suggest that histone acetylation may affect DNA and histone methylation during mitosis. Treatment with 5-azacytidine, a cytosine analog that reduces DNA methylation, caused chromatin decondensation and mediated an increase in H4 acetylation, in addition to reduced DNA methylation and H3K9 dimethylation during interphase and mitosis. These results suggest that decreased DNA methylation causes a reduction in H3K9 dimethylation and an increase in H4 acetylation.

CONCLUSIONS

The interchangeable effects of 5-azacytidine and trichostatin A on H4 acetylation, DNA methylation and H3K9 dimethylation indicate a mutually reinforcing action between histone acetylation, DNA methylation and histone methylation with respect to chromatin modification. Treatment with trichostatin A and 5-azacytidine treatment caused a decrease in the mitotic index, suggesting that H4 deacetylation and DNA and H3K9 methylation may contain the necessary information for triggering mitosis in maize root tips.

摘要

背景

各种组合的 DNA 和组蛋白修饰与许多细胞过程相关。在这项研究中，我们研究了玉米根尖分生组织有丝分裂过程中组蛋白 H4 四乙酰化、DNA 甲基化和组蛋白 H3 赖氨酸 9 二甲基化之间可能存在的关系。

结果

用抑制组蛋白去乙酰化酶的曲古抑菌素 A 处理后，导致组蛋白 H4 乙酰化增加，伴随着有丝分裂中期间期染色质去凝聚，以及整个 H3K9 二甲基化和 DNA 甲基化减少。这些观察结果表明，组蛋白乙酰化可能影响有丝分裂过程中的 DNA 和组蛋白甲基化。用 5-氮杂胞苷处理，一种减少 DNA 甲基化的胞嘧啶类似物，引起染色质去凝聚，并介导 H4 乙酰化增加，以及间期和有丝分裂过程中 DNA 甲基化和 H3K9 二甲基化减少。这些结果表明，DNA 甲基化减少导致 H3K9 二甲基化减少和 H4 乙酰化增加。

结论

5-氮杂胞苷和曲古抑菌素 A 对 H4 乙酰化、DNA 甲基化和 H3K9 二甲基化的可互换影响表明，组蛋白乙酰化、DNA 甲基化和组蛋白甲基化在染色质修饰方面存在相互增强的作用。曲古抑菌素 A 和 5-氮杂胞苷处理降低了有丝分裂指数，表明 H4 去乙酰化和 DNA 以及 H3K9 甲基化可能包含触发玉米根尖有丝分裂的必要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/a54a8cfb8e89/1471-2229-10-178-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/88fd19250ba5/1471-2229-10-178-1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/a9b15d1bb8d1/1471-2229-10-178-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/ef6b82c9cd00/1471-2229-10-178-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/a54a8cfb8e89/1471-2229-10-178-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/88fd19250ba5/1471-2229-10-178-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/ac7be16753aa/1471-2229-10-178-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/10647b4b486c/1471-2229-10-178-3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/a9b15d1bb8d1/1471-2229-10-178-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/ef6b82c9cd00/1471-2229-10-178-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bb/3095308/a54a8cfb8e89/1471-2229-10-178-7.jpg

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