[P120-连环蛋白亚型1A和3A对肺癌细胞中E-钙黏蛋白和β-连环蛋白的表达有不同影响。]
[P120-catenin Isoforms 1A and 3A Differently Affect the Expression of E-cadherin and beta-catenin in Lung Cancer Cells.].
作者信息
Fang Changqing, Liu Yang, Liu Nan, Zhang Yong, Wang Enhua
机构信息
Department of Pathology, College of Basic Medical Sciences, China Medical University and Department of Pathology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2009 Jul 20;12(7):741-6. doi: 10.3779/j.issn.1009-3419.2009.07.003.
BACKGROUND
Different isoforms of p120-catenin (p120(ctn)), the members of catenin family, are variably expressed in different tissues and cells. These isoforms may confer different properties with respect to cell adhesion and migration, but this concept needs experimental evidence.
METHODS
To more precisely define their biological roles, we stably transfected isoforms 1A and 3A into the lung cancer cell lines A549 and NCI-H460, which had low expression of p120(ctn). Using RT-PCR, Western Blot, and Transwell, we testified their impact on the expression of E-cadherin, beta-catenin, and the invasiveness of lung cancer cells.
RESULTS
Expression of isoform 1A of p120(ctn) upregulated E-cadherin and beta-catenin, as well as the ability of cells to invasion. In contrast, overexpression of isoform 3A had a smaller influence on invasion.
CONCLUSIONS
P120(ctn) isoforms 1A and 3A may differently regulate the invasive, adhesive of lung cancer cells through distinct regulation of the expression of E-cadherin and beta-catenin.
背景
连环蛋白家族成员p120连环蛋白（p120(ctn)）的不同异构体在不同组织和细胞中表达各异。这些异构体在细胞黏附和迁移方面可能具有不同特性，但这一概念需要实验证据支持。
方法
为更精确地确定其生物学作用，我们将异构体1A和3A稳定转染至p120(ctn)低表达的肺癌细胞系A549和NCI-H460中。运用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法（Western Blot）和Transwell实验，我们证实了它们对E-钙黏蛋白、β-连环蛋白表达以及肺癌细胞侵袭能力的影响。
结果
p120(ctn)异构体1A的表达上调了E-钙黏蛋白和β-连环蛋白，以及细胞的侵袭能力。相比之下，异构体3A的过表达对侵袭的影响较小。
结论
p120(ctn)异构体1A和3A可能通过对E-钙黏蛋白和β-连环蛋白表达的不同调控，以不同方式调节肺癌细胞的侵袭和黏附。
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