手性分离依康唑的胶束电动色谱法与羟丙基-γ-环糊精。

Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-gamma-cyclodextrin.

机构信息

Separation Science and Technology Group (SepSTec), Ibnu Sina Institute for Fundamental Science Studies, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.

出版信息

J Pharm Biomed Anal. 2010 Dec 15;53(5):1244-9. doi: 10.1016/j.jpba.2010.07.030. Epub 2010 Jul 30.

DOI:10.1016/j.jpba.2010.07.030

PMID:20719457

Abstract

A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method with hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as chiral selector for the enantiomeric separation of econazole is reported. Enantioseparation of econazole was successfully achieved by the optimized CD-MEKC system containing 40mM HP-gamma-CD, 50mM SDS and 20mM phosphate buffer (pH 8) solution with an analysis time of less than 9min. Calibration curves were linear for the two stereoisomers of econazole (r(2)>0.998). Good repeatabilities in the migration time, peak area and peak height were obtained in terms of RSD% ranging from 0.30 to 7.67%. Combination of solid-phase extraction (SPE) procedure using diol column and the CD-MEKC method was successfully applied to the determination of econazole in a formulated cream sample.

摘要

报道了一种以羟丙基-γ-环糊精（HP-γ-CD）为手性选择剂的环糊精修饰胶束电动色谱（CD-MEKC）方法，用于依康唑对映体的分离。在优化的 CD-MEKC 体系中，成功地实现了依康唑的对映体分离，该体系含有 40mM HP-γ-CD、50mM SDS 和 20mM 磷酸盐缓冲液（pH 8），分析时间不到 9min。两种依康唑立体异构体的校准曲线均呈线性（r(2)>0.998）。在迁移时间、峰面积和峰高方面，重复性良好，RSD% 范围为 0.30%至 7.67%。使用二醇柱的固相萃取（SPE）程序与 CD-MEKC 方法的组合成功地应用于制剂乳膏样品中依康唑的测定。