Separation Science and Technology Group, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia.
Chirality. 2012 Mar;24(3):252-4. doi: 10.1002/chir.21990. Epub 2012 Jan 24.
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique has been developed for enantioseparation of vinpocetine using an inexpensive 2-hydroxypropyl-β-CD (HP-β-CD) as the chiral selector (CS). The best chiral separation was achieved using 40 mM HP-β-CD as the CS in 50 mM phosphate buffer (pH 7.0) consisting of 40 mM sodium dodecyl sulfate (SDS) at a separation temperature and separation voltage of 25°C and 25 kV, respectively. To the author's best knowledge, this is the first CD-MEKC study able to successfully separate the four stereoisomer of vinpocetine in separation time of 9.5 min and resolution of 1.04-3.87.
建立了一种手性选择剂为廉价的 2-羟丙基-β-环糊精（HP-β-CD）的环糊精修饰胶束电动色谱（CD-MEKC）技术，用于文拉法辛对映体的拆分。在分离温度和分离电压分别为 25°C 和 25 kV 的条件下，使用 40 mM 磷酸缓冲液（pH 7.0）中含有 40 mM 十二烷基硫酸钠（SDS）和 40 mM HP-β-CD 作为手性选择剂，可实现最佳的手性分离。据作者所知，这是首例在 9.5 分钟的分离时间内成功拆分文拉法辛四个立体异构体，且分离度为 1.04-3.87 的 CD-MEKC 研究。
