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[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--filter paper method].

作者信息

Yamaguchi K, Ushijima H

机构信息

Department of Internal Medicine, Juntendo University School of Medicine.

出版信息

Kansenshogaku Zasshi. 1991 Mar;65(3):273-6. doi: 10.11150/kansenshogakuzasshi1970.65.273.

DOI:10.11150/kansenshogakuzasshi1970.65.273
PMID:2071947
Abstract

We have reported novel methods for screening of HIV by using polymerase chain reaction (PCR) and digoxigenin labelled probes. Here we developed a filter paper method for samples from long distances. About 50 microliters of peripheral bloods were absorbed in filters (5 mm diameter), dried, heated, then stored for 1 month at room temperature before PCR. This method was proved to be sufficient for detection of HIV proviruses from clinical samples.

摘要

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